|
| Status |
Public on May 13, 2019 |
| Title |
E14 mESCs CRISPR/Cas9 Site 9 replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
passages: 18-27
strain: 129/Ola
cell sex: M
|
| Treatment protocol |
Lipofection of pX CRISPR plasmids with puromycin or GFP selection
|
| Growth protocol |
Feeder-free LIF conditioned mESC growth medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen Dneasy Blood and Tissue Kit
PCR based addition of Illumina sequencing adapters
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
E14 mESCs targeted using CRISPR/Cas9 at Site 9 (Prickle2, chr6)
|
| Data processing |
Illumina MiSeq base calling
Adapter trimming using trim_galore 0.3.1
Mapping and allele reconstruction using CRISPResso 1.0.8
Genome_build: mm9
Supplementary_files_format_and_content: CRISPResso allele frequency file
|
| |
|
| Submission date |
May 02, 2019 |
| Last update date |
May 13, 2019 |
| Contact name |
Dominic Owens |
| E-mail(s) |
dominic.owens@utoronto.ca
|
| Organization name |
University of Oxford
|
| Department |
MRC WIMM
|
| Street address |
John Radcliffe Hospital
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE130621 |
Mapping of microhomologies at CRISPR/Cas9 deletions |
|
| Relations |
| BioSample |
SAMN11565517 |
| SRA |
SRX5781508 |
