|
| Status |
Public on Feb 25, 2009 |
| Title |
genotype: Mla6 - pathogen isolates: K1 - time: 32 - rep1 |
| Sample type |
RNA |
| |
|
| Source name |
##;PO:0009011## (genotype: Mla6 - pathogen isolates: K1 - time: 32)
|
| Organism |
Hordeum vulgare |
| Characteristics |
germplasm name: c.i. 16151
organism part: PO:0009011
seedling growth: GRO:0007058
development stage: first leaf unfolded GRO:0007060
age: 7 days after sowing
growth media: peat/perlite/soil
genotype: Widtype, pathogen isolate K1
time: 32, rep-1
|
| Growth protocol |
Growth Description: GH grown and inoculated in 18oC chamber
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot Phenol/Guanidine Thiocyanate and RNeasy Clean up
|
| Label |
biotin
|
| Label protocol |
Affy-Eukaryotic Target Preparation
|
| |
|
| Hybridization protocol |
Affy- Eukaryotic Target Hybridization
|
| Scan protocol |
Affy- Array Scanning
|
| Description |
Widtype, pathogen isolate K1, time 32, rep-1
|
| Data processing |
The MAS5.0 Normalization is performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org). The target value is 500 for all cases. The code was developed using R2.4.1, Bioconductor 1.9, and simpleaffy 2.8.0. The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html. The normalization scripts are available from the PLEXdb team by request.
|
| |
|
| Submission date |
Feb 20, 2009 |
| Last update date |
Feb 24, 2009 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL1340 |
| Series (1) |
| GSE14930 |
Comparison of wild-type and cell death mutant of barley plants containing Mla6 powdery mildew resistance gene |
|
