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Sample GSM372943 Query DataSets for GSM372943
Status Public on Feb 25, 2009
Title genotype: Mla6 - pathogen isolates: 5874 - time: 8 - rep3
Sample type RNA
 
Source name ##;PO:0009011## (genotype: Mla6 - pathogen isolates: 5874 - time: 8)
Organism Hordeum vulgare
Characteristics germplasm name: c.i. 16151
organism part: PO:0009011
seedling growth: GRO:0007058
development stage: first leaf unfolded GRO:0007060
age: 7 days after sowing
growth media: peat/perlite/soil
genotype: Widtype, pathogen isolate 5874
time: 8, rep-3
Growth protocol Growth Description: GH grown and inoculated in 18oC chamber
Extracted molecule total RNA
Extraction protocol Hot Phenol/Guanidine Thiocyanate and RNeasy Clean up
Label biotin
Label protocol Affy-Eukaryotic Target Preparation
 
Hybridization protocol Affy- Eukaryotic Target Hybridization
Scan protocol Affy- Array Scanning
Description Widtype, pathogen isolate 5874, time 8, rep-3
Data processing The MAS5.0 Normalization is performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org). The target value is 500 for all cases. The code was developed using R2.4.1, Bioconductor 1.9, and simpleaffy 2.8.0. The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html. The normalization scripts are available from the PLEXdb team by request.
 
Submission date Feb 20, 2009
Last update date Feb 24, 2009
Contact name PLEXdb Curator, Sudhansu Dash
Organization name Iowa State University
Department Virtual Reality Application Center
Lab PLEXdb
Street address 1023 Crop Genomes Informatics Lab (Iowa State University)
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platform ID GPL1340
Series (1)
GSE14930 Comparison of wild-type and cell death mutant of barley plants containing Mla6 powdery mildew resistance gene

Data table header descriptions
ID_REF Affymetrix probe set id
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1200459_Reg_88-1740_at 73.236 P 0.00639609
1289374_Reg_826-1545_at 10.487 A 0.638946
149174_Reg_66-1115_at 7.7387 A 0.876127
150775_Reg_211-1236_at 8.315 A 0.703144
23381122_R_1101-1898_at 140.36 A 0.296856
2623978_R_1403-2212_at 53.607 P 0.0164271
40321-46140.AF427791_x_at 0.83281 A 0.468736
48446_Reg_1231-2205_at 37.918 A 0.570568
48446_Reg_137-1204_at 3.1266 A 0.788202
74797-75570.AF427791_x_at 22.573 A 0.535424
8829-9073.AF427791_x_at 8.729 A 0.479677
9507414_R_4264-4644_at 101.94 A 0.429432
A00196.1_at 19.793 A 0.671679
A06498.1_s_at 6.8448 A 0.856998
AB011264_at 15.569 A 0.296856
AB011266_at 46.34 A 0.143002
AB019525_at 20.396 A 0.638946
AB024007_at 39.003 A 0.106612
AF016328_at 155.55 P 0.0204299
AF026538_at 163.27 P 0.0455804

Total number of rows: 22840

Table truncated, full table size 762 Kbytes.




Supplementary file Size Download File type/resource
GSM372943.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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