|
| Status |
Public on Feb 19, 2009 |
| Title |
Clone3200_LPI5_N-_8w_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
LPI5, low nitrogen, 8-week
|
| Organism |
Populus fremontii x Populus angustifolia |
| Characteristics |
clone: 3200
|
| Treatment protocol |
Plants of ~1 m height were distributed into two large ebb and flow hydroponic tubs, one receiving 5 mM nitrogen (N+) and the other with 0.125 mM nitrogen (N-). Plants were maintained as described in Growth Protocol for 8 weeks prior to sampling.
|
| Growth protocol |
Rooted cuttings, 10-15 cm in height, were transferred into aerated nutrient solution containing 5 mM nitrogen. Distilled water was added daily as needed to maintain a constant nutrient volume. Nutrient solution was replaced weekly, and a pH of 5.8±0.3 was maintained by daily additions of 2N KOH or 2N HCl.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CTAB protocol
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
Gene expression data from a 8-week N experiment.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 18, 2009 |
| Last update date |
Feb 18, 2009 |
| Contact name |
Chung-Jui Tsai |
| E-mail(s) |
cjtsai@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
School of Forestry and Natural Resources
|
| Street address |
120 Green Street
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30606 |
| Country |
USA |
| |
|
| Platform ID |
GPL4359 |
| Series (3) |
| GSE14893 |
Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 3200 |
| GSE14894 |
Comparative transcriptomics analysis of Populus leaves under nitrogen limitation |
| GSE16786 |
Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture |
|
