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Sample GSM372097 Query DataSets for GSM372097
Status Public on Feb 19, 2009
Title Clone3200_LPI5_N-_8w_rep1
Sample type RNA
Source name LPI5, low nitrogen, 8-week
Organism Populus fremontii x Populus angustifolia
Characteristics clone: 3200
Treatment protocol Plants of ~1 m height were distributed into two large ebb and flow hydroponic tubs, one receiving 5 mM nitrogen (N+) and the other with 0.125 mM nitrogen (N-). Plants were maintained as described in Growth Protocol for 8 weeks prior to sampling.
Growth protocol Rooted cuttings, 10-15 cm in height, were transferred into aerated nutrient solution containing 5 mM nitrogen. Distilled water was added daily as needed to maintain a constant nutrient volume. Nutrient solution was replaced weekly, and a pH of 5.8±0.3 was maintained by daily additions of 2N KOH or 2N HCl.
Extracted molecule total RNA
Extraction protocol CTAB protocol
Label biotin
Label protocol Affymetrix
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Gene expression data from a 8-week N experiment.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Feb 18, 2009
Last update date Feb 18, 2009
Contact name Chung-Jui Tsai
Organization name University of Georgia
Department School of Forestry and Natural Resources
Street address 120 Green Street
City Athens
State/province GA
ZIP/Postal code 30606
Country USA
Platform ID GPL4359
Series (3)
GSE14893 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 3200
GSE14894 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation
GSE16786 Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-3_at 1010.8 P 0.000044
AFFX-BioB-5_at 1024 P 0.000509
AFFX-BioB-M_at 1597.7 P 0.000044
AFFX-BioC-3_at 3127 P 0.000044
AFFX-BioC-5_at 3305 P 0.000044
AFFX-BioDn-3_at 12284.9 P 0.000044
AFFX-BioDn-5_at 7077.7 P 0.000044
AFFX-CreX-3_at 34224.9 P 0.000044
AFFX-CreX-5_at 31458.7 P 0.000052
AFFX-DapX-3_at 36.3 A 0.175328
AFFX-DapX-5_at 11.8 A 0.699394
AFFX-DapX-M_at 9 A 0.672921
AFFX-LysX-3_at 36.2 A 0.083592
AFFX-LysX-5_at 32.4 A 0.216504
AFFX-LysX-M_at 7.3 A 0.897855
AFFX-PheX-3_at 8.6 A 0.772364
AFFX-PheX-5_at 10.1 A 0.783496
AFFX-PheX-M_at 5.3 A 0.834139
AFFX-Ptp-actin-3_s_at 17628.5 P 0.000244
AFFX-Ptp-actin-5_a_at 12846.7 P 0.008057

Total number of rows: 61413

Table truncated, full table size 2296 Kbytes.

Supplementary file Size Download File type/resource
GSM372097.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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