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Sample GSM372095 Query DataSets for GSM372095
Status Public on Feb 19, 2009
Title Clone3200_LPI5_N-_4w_rep1
Sample type RNA
 
Source name LPI5, low nitrogen, 4-week
Organism Populus fremontii x Populus angustifolia
Characteristics clone: 3200
Treatment protocol Plants of ~1 m height were distributed into two large ebb and flow hydroponic tubs, one receiving 5 mM nitrogen (N+) and the other with 0.125 mM nitrogen (N-). Plants were maintained as described in Growth Protocol for 4 weeks prior to sampling.
Growth protocol Rooted cuttings, 10-15 cm in height, were transferred into aerated nutrient solution containing 5 mM nitrogen. Distilled water was added daily as needed to maintain a constant nutrient volume. Nutrient solution was replaced weekly, and a pH of 5.8±0.3 was maintained by daily additions of 2N KOH or 2N HCl.
Extracted molecule total RNA
Extraction protocol CTAB protocol
Label biotin
Label protocol Affymetrix
 
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Gene expression data from a 4-week N experiment.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Feb 18, 2009
Last update date Feb 18, 2009
Contact name Chung-Jui Tsai
E-mail(s) cjtsai@uga.edu
Organization name University of Georgia
Department School of Forestry and Natural Resources
Street address 120 Green Street
City Athens
State/province GA
ZIP/Postal code 30606
Country USA
 
Platform ID GPL4359
Series (3)
GSE14893 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 3200
GSE14894 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation
GSE16786 Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 2686.2 P 0.000044
AFFX-BioB-5_at 2385.6 P 0.00034
AFFX-BioB-M_at 4129 P 0.000044
AFFX-BioC-3_at 2042.9 P 0.000044
AFFX-BioC-5_at 2161.8 P 0.000052
AFFX-BioDn-3_at 29362.8 P 0.000044
AFFX-BioDn-5_at 17413.9 P 0.000044
AFFX-CreX-3_at 80625.5 P 0.000044
AFFX-CreX-5_at 67698 P 0.000052
AFFX-DapX-3_at 1678.3 P 0.000195
AFFX-DapX-5_at 484.1 P 0.011384
AFFX-DapX-M_at 1160.5 P 0.00039
AFFX-LysX-3_at 167.1 P 0.042962
AFFX-LysX-5_at 124.7 A 0.116113
AFFX-LysX-M_at 118.3 A 0.645547
AFFX-PheX-3_at 101.2 A 0.48511
AFFX-PheX-5_at 157.9 P 0.039661
AFFX-PheX-M_at 63.9 A 0.368438
AFFX-Ptp-actin-3_s_at 23678 P 0.000244
AFFX-Ptp-actin-5_a_at 15997.5 P 0.000244

Total number of rows: 61413

Table truncated, full table size 2308 Kbytes.




Supplementary file Size Download File type/resource
GSM372095.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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