|
| Status |
Public on Jan 19, 2021 |
| Title |
WT3 |
| Sample type |
SRA |
| |
|
| Source name |
wild-type_splenic Tfh cells
|
| Organism |
Mus musculus |
| Characteristics |
stain background: C57BL/6J
genotype/variation: wild-type
cell type: splenic Tfh cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tfh cells (SLAM-loCD44+CD62L-CD4+) were directly sorted into TRIzol reagent by a FACSAriaII sorter, and total RNA was extracted using standard Qiagen RNease columns.
RNA libraries were prepared for sequencing using standard Illumina protocols。
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
The quality of RNA-Seq libraries was assessed by FastQC (v0.11.5).
RNA-Seq libraries were mapped to mouse genome mm10 (version M17) using TopHat (v2.1.1).
Expression levels of all genes were estimated by HTseq (v0.6.1), and Reads Per Kilobase per Million mapped reads (RPKM) counts were calculated.
Genome_build: mm10
Supplementary_files_format_and_content: abundance measurements
|
| |
|
| Submission date |
Apr 11, 2019 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Shuyang Yu |
| E-mail(s) |
ysy@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Department |
College of Biological Sciences
|
| Street address |
2 Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE129648 |
Regulation of Tfh differentiation by Mettl3 [RNA-seq] |
| GSE129650 |
m6A methyltransferase METTL3 regulates Tfh cell differentiation |
|
| Relations |
| BioSample |
SAMN11397992 |
| SRA |
SRX5668322 |
