|
| Status |
Public on Mar 19, 2019 |
| Title |
Human cochlea |
| Sample type |
RNA |
| |
|
| Source name |
Healthy inner ear
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: basilar membrane, lateral wall, modiolus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent (Thermofisher, cat #15596018) and purified by centrifuging with phase lock heavy gel (Tiagen, cat # WMS-2302830
|
| Label |
biotin
|
| Label protocol |
Using an Affymetrix GeneChip WT Pico labelling kit, 50 ng of total RNA was TdT end labelled with biotin
|
| |
|
| Hybridization protocol |
Affymetrix HTA 2.0 array, performed at KUMC Microarray facility
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G using a single scan with default normalization
|
| Description |
RNA expression derived from Affymetrix console
|
| Data processing |
affymetrix-algorithm-name = rma-gene-full
affymetrix-algorithm-version = 1.0
affymetrix-array-type = HTA-2_0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Mar 18, 2019 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Hinrich Staecker |
| E-mail(s) |
hstaecker@kumc.edu
|
| Organization name |
University of Kansas School of Medicine
|
| Department |
Otolaryngology Head and Neck Surgery
|
| Street address |
Rainbow Blvd.
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
|
