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Sample GSM363778 Query DataSets for GSM363778
Status Public on Jan 27, 2009
Title miR-124 transfection lysate RNA, biological replicate 2
Sample type RNA
 
Source name miR-124 transfection 2, total RNA
Organism Homo sapiens
Characteristics FLPin HEK 293 cells stably transfected with FLAG/HA-AGO2
Treatment protocol FLAG/HA-AGO2 was immunoprecipitated with anti-FLAG M2 agarose
Growth protocol HEK293 cells were grown in DMEM suppl. with FBS and 2mM Glutamine
Extracted molecule total RNA
Extraction protocol After proteinase K treatment extraction of total RNA was performed using Trizol.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix One-Cycle Eukaryotic Target Labeling Assay protocol from total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from total RNA of mock-transfected cells
Data processing CEL files were imported into R / BioConductor using the affy package. The raw intensities were corrected using gcRMA. The 18 arrays were finally quantile-normalized.
 
Submission date Jan 23, 2009
Last update date Jan 26, 2009
Contact name Markus Landthaler
E-mail(s) landthm@rockefeller.edu
Organization name Rockefeller University
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL570
Series (1)
GSE14537 Contribution of sequence and structure to mRNA-binding of Argonaute/miRNA complexes and degradation of miRNA targets

Data table header descriptions
ID_REF
VALUE gcRMA normalized signal

Data table
ID_REF VALUE
1007_s_at 7.89118653343923
1053_at 10.4282425011752
117_at 2.24903990891081
121_at 2.65316578250214
1255_g_at 2.20821013899568
1294_at 2.20821013899568
1316_at 2.2703179030543
1320_at 2.20821013899568
1405_i_at 2.20821013899568
1431_at 2.20821013899568
1438_at 2.21502035775897
1487_at 6.51406107963802
1494_f_at 2.20821013899568
1552256_a_at 11.4719776489198
1552257_a_at 11.0483631848376
1552258_at 2.20821013899568
1552261_at 2.20821013899568
1552263_at 7.48856569334783
1552264_a_at 8.2924533046347
1552266_at 2.20821013899568

Total number of rows: 54675

Table truncated, full table size 1480 Kbytes.




Supplementary file Size Download File type/resource
gsm363778.cel.gz 8.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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