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Sample GSM362889 Query DataSets for GSM362889
Status Public on Jan 04, 2010
Title Clone1979_LPI5_N-_4w_rep1
Sample type RNA
Source name LPI5, low nitrogen, 4-week
Organism Populus fremontii x Populus angustifolia
Characteristics clone: 1979
Treatment protocol Plants of ~1 m height were distributed into two large ebb and flow hydroponic tubs, one receiving 5 mM nitrogen (N+) and the other with 0.125 mM nitrogen (N-). Plants were maintained as described in Growth Protocol for 4 weeks prior to sampling
Growth protocol Rooted cuttings, 10-15 cm in height, were transferred into aerated nutrient solution containing 5 mM nitrogen. Distilled water was added daily as needed to maintain a constant nutrient volume. Nutrient solution was replaced weekly, and a pH of 5.8±0.3 was maintained by daily additions of 2N KOH or 2N HCl.
Extracted molecule total RNA
Extraction protocol CTAB protocol
Label biotin
Label protocol Affymetrix
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Gene expression data from a 4-week N experiment
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Jan 22, 2009
Last update date Jan 04, 2010
Contact name Chung-Jui Tsai
Organization name University of Georgia
Department School of Forestry and Natural Resources
Street address 120 Green Street
City Athens
State/province GA
ZIP/Postal code 30606
Country USA
Platform ID GPL4359
Series (3)
GSE14515 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 1979
GSE14894 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation
GSE16786 Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-3_at 2303.5 P 0.000044
AFFX-BioB-5_at 1855.2 P 0.000147
AFFX-BioB-M_at 3380.6 P 0.000044
AFFX-BioC-3_at 1912.8 P 0.000052
AFFX-BioC-5_at 2280.4 P 0.000052
AFFX-BioDn-3_at 26878.4 P 0.000044
AFFX-BioDn-5_at 15774.4 P 0.000044
AFFX-CreX-3_at 76477.9 P 0.000044
AFFX-CreX-5_at 62058.1 P 0.000052
AFFX-DapX-3_at 2700.5 P 0.000095
AFFX-DapX-5_at 721.4 P 0.000127
AFFX-DapX-M_at 1815.6 P 0.000195
AFFX-LysX-3_at 257.4 P 0.003212
AFFX-LysX-5_at 172.8 P 0.030976
AFFX-LysX-M_at 130.3 A 0.205732
AFFX-PheX-3_at 156.5 A 0.156732
AFFX-PheX-5_at 133.6 A 0.287743
AFFX-PheX-M_at 198.5 P 0.030976
AFFX-Ptp-actin-3_s_at 28252.2 P 0.000244
AFFX-Ptp-actin-5_a_at 10408.3 P 0.000732

Total number of rows: 61413

Table truncated, full table size 2308 Kbytes.

Supplementary file Size Download File type/resource
GSM362889.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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