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Sample GSM362886 Query DataSets for GSM362886
Status Public on Jan 04, 2010
Title Clone1979_LPI2_N-_4w_rep2
Sample type RNA
Source name LPI2, low nitrogen, 4-week
Organism Populus fremontii x Populus angustifolia
Characteristics clone: 1979
Treatment protocol Plants of ~1 m height were distributed into two large ebb and flow hydroponic tubs, one receiving 5 mM nitrogen (N+) and the other with 0.125 mM nitrogen (N-). Plants were maintained as described in Growth Protocol for 4 weeks prior to sampling
Growth protocol Rooted cuttings, 10-15 cm in height, were transferred into aerated nutrient solution containing 5 mM nitrogen. Distilled water was added daily as needed to maintain a constant nutrient volume. Nutrient solution was replaced weekly, and a pH of 5.8±0.3 was maintained by daily additions of 2N KOH or 2N HCl.
Extracted molecule total RNA
Extraction protocol CTAB protocol
Label biotin
Label protocol Affymetrix
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Gene expression data from a 4-week N experiment
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Jan 22, 2009
Last update date Jan 04, 2010
Contact name Chung-Jui Tsai
Organization name University of Georgia
Department School of Forestry and Natural Resources
Street address 120 Green Street
City Athens
State/province GA
ZIP/Postal code 30606
Country USA
Platform ID GPL4359
Series (3)
GSE14515 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 1979
GSE14894 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation
GSE16786 Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-3_at 2670.8 P 0.000044
AFFX-BioB-5_at 2118.1 P 0.000095
AFFX-BioB-M_at 3687.6 P 0.000044
AFFX-BioC-3_at 1970 P 0.000052
AFFX-BioC-5_at 2222.9 P 0.000052
AFFX-BioDn-3_at 31181.5 P 0.000044
AFFX-BioDn-5_at 16710.9 P 0.000044
AFFX-CreX-3_at 88073.4 P 0.000044
AFFX-CreX-5_at 77309.1 P 0.000052
AFFX-DapX-3_at 1328.5 P 0.00011
AFFX-DapX-5_at 481.1 P 0.001102
AFFX-DapX-M_at 1284.2 P 0.00034
AFFX-LysX-3_at 23.1 A 0.58862
AFFX-LysX-5_at 146.7 A 0.131361
AFFX-LysX-M_at 110.5 A 0.544587
AFFX-PheX-3_at 196.5 P 0.020022
AFFX-PheX-5_at 157.6 M 0.050229
AFFX-PheX-M_at 226.9 P 0.026111
AFFX-Ptp-actin-3_s_at 17243.5 P 0.000244
AFFX-Ptp-actin-5_a_at 12906.2 P 0.000244

Total number of rows: 61413

Table truncated, full table size 2308 Kbytes.

Supplementary file Size Download File type/resource
GSM362886.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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