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Sample GSM362885 Query DataSets for GSM362885
Status Public on Jan 04, 2010
Title Clone1979_LPI2_N-_4w_rep1
Sample type RNA
 
Source name LPI2, low nitrogen, 4-week
Organism Populus fremontii x Populus angustifolia
Characteristics clone: 1979
Treatment protocol Plants of ~1 m height were distributed into two large ebb and flow hydroponic tubs, one receiving 5 mM nitrogen (N+) and the other with 0.125 mM nitrogen (N-). Plants were maintained as described in Growth Protocol for 4 weeks prior to sampling
Growth protocol Rooted cuttings, 10-15 cm in height, were transferred into aerated nutrient solution containing 5 mM nitrogen. Distilled water was added daily as needed to maintain a constant nutrient volume. Nutrient solution was replaced weekly, and a pH of 5.8±0.3 was maintained by daily additions of 2N KOH or 2N HCl.
Extracted molecule total RNA
Extraction protocol CTAB protocol
Label biotin
Label protocol Affymetrix
 
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Gene expression data from a 4-week N experiment
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 22, 2009
Last update date Jan 04, 2010
Contact name Chung-Jui Tsai
E-mail(s) cjtsai@uga.edu
Organization name University of Georgia
Department School of Forestry and Natural Resources
Street address 120 Green Street
City Athens
State/province GA
ZIP/Postal code 30606
Country USA
 
Platform ID GPL4359
Series (3)
GSE14515 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 1979
GSE14894 Comparative transcriptomics analysis of Populus leaves under nitrogen limitation
GSE16786 Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 1104.8 P 0.000044
AFFX-BioB-5_at 927.2 P 0.000509
AFFX-BioB-M_at 1432.2 P 0.000044
AFFX-BioC-3_at 991.8 P 0.00007
AFFX-BioC-5_at 1035.8 P 0.000095
AFFX-BioDn-3_at 13452.9 P 0.000044
AFFX-BioDn-5_at 7472.6 P 0.000044
AFFX-CreX-3_at 42559.2 P 0.000044
AFFX-CreX-5_at 34643.5 P 0.000052
AFFX-DapX-3_at 1673.6 P 0.000147
AFFX-DapX-5_at 463.6 P 0.001248
AFFX-DapX-M_at 1437.1 P 0.000225
AFFX-LysX-3_at 279.2 P 0.00034
AFFX-LysX-5_at 60.7 A 0.239063
AFFX-LysX-M_at 156.2 A 0.227636
AFFX-PheX-3_at 216 A 0.147939
AFFX-PheX-5_at 106.3 P 0.00844
AFFX-PheX-M_at 164.7 P 0.01667
AFFX-Ptp-actin-3_s_at 22512.3 P 0.000244
AFFX-Ptp-actin-5_a_at 13118.8 P 0.000244

Total number of rows: 61413

Table truncated, full table size 2305 Kbytes.




Supplementary file Size Download File type/resource
GSM362885.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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