|
| Status |
Public on Oct 18, 2019 |
| Title |
Green receptacle R2 |
| Sample type |
RNA |
| |
|
| Source name |
Strawberry green unripen receptacle replicate 2
|
| Organism |
Fragaria x ananassa |
| Characteristics |
tissue: Inmature fruits
|
| Treatment protocol |
Fruits were harvested in green and red stages.
|
| Growth protocol |
Strawberry plants (F. x ananassa) were grown in plant chamber at 25 ºC, 10.000 lux and 80% humidity and afterwards maintained in a greenhouse.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from independent strawberry fruits as stated by Asif et al. (2000). The total RNA obtained was treated with DNase I (RNase free) (Invitrogen), in accordance with the manufacturer's instructions, to remove genomic DNA contamination, after which it was purified by using the RNeasy Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
200 ng of total RNA of each sample was labelled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
600 ng of Cy3-labeled aRNA was hybridized on an 8x60K Sure Print G3 custom microarray (Agilent Technologies). After hybridization, arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Arrays were scanned with an Agilent Microarray Scanner. Images and intensity data were obtained using Agilent Feature Extraction v.10.7 software.
|
| Data processing |
The microarray datas were analyzed with the software for gene expression analysis ArrayStar 5 (DNASTAR).
|
| |
|
| Submission date |
Feb 07, 2019 |
| Last update date |
Oct 20, 2019 |
| Contact name |
Francisco Javier Molina-Hidalgo |
| Organization name |
University of Cordoba
|
| Department |
Biochemistry and molecular biology
|
| Street address |
Edif. Severo Ochoa (C6.) Planta Baja-Ala Norte Campus de Rabanales
|
| City |
CÓRDOBA |
| State/province |
Cordoba |
| ZIP/Postal code |
14071 |
| Country |
Spain |
| |
|
| Platform ID |
GPL26165 |
| Series (1) |
| GSE126220 |
Transcriptomic studies in the development and ripening of strawberry fruits (Agilent) |
|
