|
| Status |
Public on Oct 18, 2019 |
| Title |
PRE1_RNAi PRE1-RNAi-A1 (D)-Cy3 |
| Sample type |
RNA |
| |
|
| Source name |
Strawberry fruit agroinjected with the FaPRE1-RNAi construct
|
| Organism |
Fragaria x ananassa |
| Characteristics |
genotype/variation: overexpressing FaPRE1
|
| Treatment protocol |
The A. tumefaciens strain AGL0 (Lazo et al., 1991), containing the pFRN-FaPRE1 was used to inject the strawberry fruit receptacles (Fragaria × ananassa), according to Hoffmann et al. (2006). A total of 15-25 strawberry plants and 30-40 agroinfiltrated fruits were inoculated and analysed, respectively. As control, fruits agroinfiltrated with the empty pFRN vector were used.
|
| Growth protocol |
Strawberry plants (F. x ananassa) used for agro-infiltration were grown in plant chamber at 25 ºC, 10.000 lux and 80% humidity and afterwards maintained in a greenhouse.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from independent strawberry floral buds, as stated by Asif et al. (2000). The total RNA obtained was treated with DNase I (RNase free) (Invitrogen), in accordance with the manufacturer's instructions, to remove genomic DNA contamination, after which it was purified by using the RNeasy Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
4 µg of total RNA were label following the Two-color microarray-bases Gen Expression Analysis v. 6.5 (Agilent)
|
| |
|
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version 10.7).
|
| Description |
raw data file: D.txt
FaPRE1-RNAi R1
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Feb 01, 2019 |
| Last update date |
Oct 18, 2019 |
| Contact name |
Francisco Javier Molina-Hidalgo |
| Organization name |
University of Cordoba
|
| Department |
Biochemistry and molecular biology
|
| Street address |
Edif. Severo Ochoa (C6.) Planta Baja-Ala Norte Campus de Rabanales
|
| City |
CÓRDOBA |
| State/province |
Cordoba |
| ZIP/Postal code |
14071 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24234 |
| Series (1) |
| GSE125995 |
Role of Fragaria FaPRE1 in fruit ripening [FaPRE1-RNAi] |
|
