|
| Status |
Public on Jan 08, 2009 |
| Title |
14-day-old seedling, heat shock, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
14-day-old rice seedlings, heat shock treatment
|
| Organism |
Oryza sativa |
| Characteristics |
Japonica, variety: ZhongHua 11
|
| Treatment protocol |
42 degree celsius for 3 h
|
| Growth protocol |
Seedlings were grown in a growth chamber with a daily photoperiodic cycle of 14 h light and 10 h dark at about 28-30 degree celsius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Trizol, according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree celsius on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000
|
| Description |
14-day-old light-grown rice seedlings
|
| Data processing |
The data were analyzed with GeneChipOperating Software (1.4) using Affymetrix default analysis settings and global scaling as normalization method. The mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 04, 2009 |
| Last update date |
Jan 06, 2009 |
| Contact name |
Bin Han |
| E-mail(s) |
bhan@ncgr.ac.cn
|
| Organization name |
National Center for Gene Research，Chinese Academy of Sciences
|
| Street address |
500 Cao Bao Road
|
| City |
Shanghai |
| ZIP/Postal code |
200233 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE14275 |
Expression data for heat shock in rice seedlings |
|
