|
| Status |
Public on Jan 01, 2009 |
| Title |
V-M10 Monarch Butterfly Fall Vehicle Treated Male 10 |
| Sample type |
RNA |
| |
|
| Source name |
Fall Vehicle Treated Male
|
| Organism |
Danaus plexippus |
| Characteristics |
dissected brain
Wild caught 10/30/07
|
| Treatment protocol |
14 days Light/Dark cycle
Lights on: 7:00 AM
Lights off: 6:00 PM
Temperature (light): 23 oC
Temperature (dark): 12 oC
topical treatment of vehicle (acetone) on day 1 and day 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted using Qiagen RNeasy Mini kit
Amplified using Nugen Ovation kit
|
| Label |
CY3
|
| Label protocol |
Nimblegen commercial protocol
|
| |
|
| Hybridization protocol |
Nimblegen commercial protocol
|
| Scan protocol |
Nimblegen commercial protocol
|
| Description |
Nimblegen commercial protocol is located at http://www.nimblegen.com/products/lit/exp_userguide_2008_05_21.pdf
|
| Data processing |
Nimblegen commercial protocol
|
| |
|
| Submission date |
Dec 18, 2008 |
| Last update date |
Dec 23, 2008 |
| Contact name |
Steven Reppert |
| E-mail(s) |
steven.reppert@umassmed.edu
|
| Phone |
508-856-6148
|
| Fax |
508-856-6266
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Neurobiology
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL7829 |
| Series (1) |
| GSE14041 |
Defining Behavioral and Molecular Differences Between Summer and Migratory Monarch Butterflies |
|
