|
Status |
Public on Jul 31, 2019 |
Title |
4597T tumor unmatched |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
laryngeal carcinoma labeled with Cyanine-3 (green)
|
Organism |
Homo sapiens |
Characteristics |
tissue: squamous cell carcinoma gender: female age: 63.0 histological variant: transglottic
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from 35 LSCC and 5 normal pool laryngeal tissue from total laryngectomy was isolated by the standard Rneasy or Trizol-based method.
|
Label |
Cy3
|
Label protocol |
Tumor and reference samples (300ng) were differentially labeled using Low Input Quick Amp Labeling (Agilent) (Agilent Technologies, Santa Clara, CA, USA).
|
|
|
Channel 2 |
Source name |
pool of 5 adjacent normal laryngeal tissue labeled with Cyanine-5 (red)
|
Organism |
Homo sapiens |
Characteristics |
sample type: reference tissue: normal laryngeal tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from 35 LSCC and 5 normal pool laryngeal tissue from total laryngectomy was isolated by the standard Rneasy or Trizol-based method.
|
Label |
Cy5
|
Label protocol |
Tumor and reference samples (300ng) were differentially labeled using Low Input Quick Amp Labeling (Agilent) (Agilent Technologies, Santa Clara, CA, USA).
|
|
|
|
Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
Scan protocol |
Images were capture in Microarray Scanner System (Agilent), with Scan Control Software 8.1 (Agilent). Images were quantified using Agilent Feature Extraction Software (version v.10.1.1.1).
|
Description |
4597TUS83203532_252800412968_S01_GE2_107_ target sample Cy3-labeled mixed with reference sample Cy5-labeled
|
Data processing |
Numeric data was generated and normalized by intensity-dependent global normalization (LOWESS) with Feature Extraction v10.1 software (Agilent)
|
|
|
Submission date |
Dec 17, 2018 |
Last update date |
Aug 02, 2019 |
Contact name |
Rainer Marco Lopez Lapa |
E-mail(s) |
marcochanca04@gmail.com
|
Phone |
+5511982849724
|
Organization name |
UNESP
|
Department |
Neogene
|
Lab |
Neogene
|
Street address |
Av. Prof. Mário Rubens Guimarães Montenegro, s/n, s/n
|
City |
Botucatu |
State/province |
SP |
ZIP/Postal code |
18618-687 |
Country |
Brazil |
|
|
Platform ID |
GPL14550 |
Series (2) |
GSE123986 |
Integrated miRNA and mRNA expression analysis uncovers drug targets in squamous cell carcinoma patients [mRNA] |
GSE124679 |
Integrated miRNA and mRNA expression analysis uncovers drug targets in squamous cell carcinoma patients |
|