|
| Status |
Public on Jul 31, 2019 |
| Title |
4471T tumor unmatched |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
laryngeal carcinoma labeled with Cyanine-3 (green)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: squamous cell carcinoma
gender: male
age: 74.0
histological variant: supraglottic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 35 LSCC and 5 normal pool laryngeal tissue from total laryngectomy was isolated by the standard Rneasy or Trizol-based method.
|
| Label |
Cy3
|
| Label protocol |
Tumor and reference samples (300ng) were differentially labeled using Low Input Quick Amp Labeling (Agilent) (Agilent Technologies, Santa Clara, CA, USA).
|
| |
|
| Channel 2 |
| Source name |
pool of 5 adjacent normal laryngeal tissue labeled with Cyanine-5 (red)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
tissue: normal laryngeal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 35 LSCC and 5 normal pool laryngeal tissue from total laryngectomy was isolated by the standard Rneasy or Trizol-based method.
|
| Label |
Cy5
|
| Label protocol |
Tumor and reference samples (300ng) were differentially labeled using Low Input Quick Amp Labeling (Agilent) (Agilent Technologies, Santa Clara, CA, USA).
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Images were capture in Microarray Scanner System (Agilent), with Scan Control Software 8.1 (Agilent).
Images were quantified using Agilent Feature Extraction Software (version v.10.1.1.1).
|
| Description |
4471TUS83203532_252800412967_S01_GE2_107_
target sample Cy3-labeled mixed with reference sample Cy5-labeled
|
| Data processing |
Numeric data was generated and normalized by intensity-dependent global normalization (LOWESS) with Feature Extraction v10.1 software (Agilent)
|
| |
|
| Submission date |
Dec 17, 2018 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Rainer Marco Lopez Lapa |
| E-mail(s) |
marcochanca04@gmail.com
|
| Phone |
+5511982849724
|
| Organization name |
UNESP
|
| Department |
Neogene
|
| Lab |
Neogene
|
| Street address |
Av. Prof. Mário Rubens Guimarães Montenegro, s/n, s/n
|
| City |
Botucatu |
| State/province |
SP |
| ZIP/Postal code |
18618-687 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE123986 |
Integrated miRNA and mRNA expression analysis uncovers drug targets in squamous cell carcinoma patients [mRNA] |
| GSE124679 |
Integrated miRNA and mRNA expression analysis uncovers drug targets in squamous cell carcinoma patients |
|
