|
| Status |
Public on Dec 14, 2019 |
| Title |
rat-model group-repF8 |
| Sample type |
RNA |
| |
|
| Source name |
rat-model group-kidney
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
sample group: doxorubicin nephropathy model
tissue: renal tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Ligation Mix Biotin (Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, mirRNA targets were hybridized for 16 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Command Console (AGCC)
|
| Description |
miRNA expression data from model group
|
| Data processing |
The data were preprocessed with R/Bioconductor package Affy ,and the processing algorithm used Range Migration Algorithm (RMA)
|
| |
|
| Submission date |
Dec 13, 2018 |
| Last update date |
Dec 15, 2019 |
| Contact name |
Xiulan Wang |
| E-mail(s) |
wangxl_1203@163.com
|
| Organization name |
Inner Mongolia University For The Nationlities
|
| Department |
Affiliated Hospital
|
| Street address |
HuoLinhe
|
| City |
Tongliao |
| State/province |
Inner Mongolia |
| ZIP/Postal code |
028000 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE123776 |
microRNA expression profile in renal tissues of rats with doxorubicin nephropathy by Mongolian medicine Narenmandula |
|
