|
| Status |
Public on Dec 03, 2018 |
| Title |
fgf3 mutant |
| Sample type |
SRA |
| |
|
| Source name |
neuromast
|
| Organism |
Danio rerio |
| Characteristics |
age: 5dpf
tissue: neuromast
genotype: fgf3-/-
strain: fgf3^t26212;ET(krt4:EGFP)SqGw57A;Tg(pou4f3:GAP-GFP)s356t (screened for mutants)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Presumptive neuromast cells (GFP+) were isolated by FACS.
Standard 10x genomics scRNA-seq protocol using v2 chemistry
Droplet based scRNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome (GRCz10) using the Cell Ranger (version 2.1.1) pipeline from 10x genomics using the default settings.
Genome_build: danRer10
Supplementary_files_format_and_content: h5 files contain the UMI counts for each cell.
|
| |
|
| Submission date |
Dec 02, 2018 |
| Last update date |
Dec 04, 2018 |
| Contact name |
Tatjana Piotrowski |
| E-mail(s) |
pio@stowers.org
|
| Organization name |
Stowers Institute
|
| Street address |
1000 E 50th St
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platform ID |
GPL18413 |
| Series (1) |
| GSE123241 |
Single cell RNA-Seq reveals Fgf signaling dynamics during sensory hair cell regeneration |
|
| Relations |
| BioSample |
SAMN10516225 |
| SRA |
SRX5087288 |
