|
| Status |
Public on Jun 06, 2019 |
| Title |
RNAseq in zebrafish wild type embryos at 36hpf replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
whole embryo
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: 36hpf
genotype: WT
|
| Growth protocol |
Embryos were cultivated at 28°C in E3 medium until desired developmental stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIsure (Bioline) and treated with TURBO DNA free kit (Invitrogen)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
RNA_seq
|
| Data processing |
Reads were aligned against reference genome using STAR software, and reads per gene were counted using htseq-count script from HTSeq tools software package
Genome_build: Zebrafish September 2014 (GRCz10/danRer10)
Supplementary_files_format_and_content: Individual counts files (reads per gene)
|
| |
|
| Submission date |
Nov 28, 2018 |
| Last update date |
Jun 06, 2019 |
| Contact name |
Juan J. Tena |
| E-mail(s) |
jjtenagu@upo.es
|
| Organization name |
CABD/CSIC
|
| Street address |
Univ. Pablo Olavide, Ctra. Utrera km1
|
| City |
Sevilla |
| ZIP/Postal code |
41013 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24776 |
| Series (2) |
| GSE123058 |
Pioneer and repressive functions of p63 during embryonic ectoderm specification [RNA-seq] |
| GSE123059 |
Pioneer and repressive functions of p63 during embryonic ectoderm specification |
|
| Relations |
| BioSample |
SAMN10492800 |
| SRA |
SRX5074432 |
