|
| Status |
Public on Jul 09, 2019 |
| Title |
PTX rep3 |
| Sample type |
SRA |
| |
|
| Source name |
skin
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hypertrophic scar fibroblasts
tissue: skin
treatment: pentoxifylline
|
| Treatment protocol |
Cells were starved for 12h and then treated with pentoxifylline for 24h
|
| Growth protocol |
Cells were cultured with DMEM/Low Glucose medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
Ion Proton Sequencing Protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
Mapsplice v2.2.0 mapping to reference genome
count counting
Genome_build: GRCh38
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Nov 25, 2018 |
| Last update date |
Jul 09, 2019 |
| Contact name |
fangfang yang |
| E-mail(s) |
cy1212@outlook.com
|
| Organization name |
xijing hospital
|
| Street address |
No. 127 Changle West Road
|
| City |
xi’an |
| ZIP/Postal code |
710032 |
| Country |
China |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE122891 |
Pentoxifylline-induced differentially-expressed genes in hypertrophic scar fibroblasts |
|
| Relations |
| BioSample |
SAMN10474382 |
| SRA |
SRX5059159 |
