|
| Status |
Public on Dec 21, 2008 |
| Title |
H9 ESC |
| Sample type |
RNA |
| |
|
| Source name |
Human Embryonic Stem Cells
|
| Organism |
Homo sapiens |
| Characteristics |
H9 passage 50 Human Embryonic Stem Cells
|
| Treatment protocol |
Lentiviral transduction of fibroblast cells was performed as previously described.
|
| Growth protocol |
iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
|
| Label |
biotin
|
| Label protocol |
Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
|
| Scan protocol |
AFX GC3000 G7 scanner
|
| Description |
Human Embryonic Stem Cell line
|
| Data processing |
RMA
|
| |
|
| Submission date |
Dec 04, 2008 |
| Last update date |
Aug 15, 2013 |
| Contact name |
Victor Ruotti |
| E-mail(s) |
ruotti@wisc.edu
|
| Phone |
608-890-0182
|
| Fax |
608-890-0167
|
| Organization name |
UW Madison
|
| Department |
Biotechnology
|
| Lab |
Thomson's Lab
|
| Street address |
425 Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13828 |
Induced pluripotent stem cells from a spinal muscular atrophy patient |
|
| Relations |
| Reanalyzed by |
GSE49910 |
