NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3466987 Query DataSets for GSM3466987
Status Public on Dec 31, 2019
Title primary bovine trophoblast giant cells from cow 4
Sample type RNA
 
Source name primary bovine trophoblast giant cells
Organism Bos taurus
Characteristics cell type: primary bovine trophoblast giant cells
Treatment protocol Primary UTC and TGC isolates were analyzed without further treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using the NucleoSpin RNA Kit including removal of genomic DNA with RNAse free rDNAse (Macherey-Nagel).
Label Biotin
Label protocol RNA from each cell sample was used to generate single strand DNA (Ambion WT Expression Kit). Fragmentation and labeling was performed with the Affymetrix Gene Chip WT Terminal Labeling and Hybridization Kit
 
Hybridization protocol The RNA was hybridized overnight (45°C) in the GeneChipR Hybridization Oven (Affymetrix) to the Affymetrix Bovine Gene 1.0 ST arrays. Washing and staining were performed according to the Affymetrix standard protocol.
Scan protocol The scanning of the microarray was done with the GeneChip Scanner 3000 7G (Affymetrix) and image processing was done with the software AGCC (Affymetrix).
Data processing Raw data were processed with the Expression Console (V1.4.1.46; Affymetrix), where normalization, background reduction and a gene-level summary was done using the RMA method (Robust Multichip Average).
 
Submission date Nov 13, 2018
Last update date Jan 24, 2020
Contact name Rainer Fuerbass
E-mail(s) fuerbass@fbn-dummerstorf.de
Organization name Leibniz Institute for Farm Animal Biology
Street address Wilhelm-Stahl-Allee
City Dummerstorf
ZIP/Postal code 18196
Country Germany
 
Platform ID GPL16500
Series (1)
GSE122474 Transcriptome analysis of bovine uninucleated trophoblast cells (UTC) and Trophoblast Giant Cells (TGC)

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
12678162 1.972753
12678166 3.044643
12678175 1.728464
12678178 3.685478
12678183 3.674594
12678186 3.636243
12678189 7.299854
12678219 1.884079
12678223 3.560821
12678232 4.484393
12678235 4.234725
12678240 3.951365
12678244 7.751688
12678246 6.018725
12678251 3.271431
12678254 4.51283
12678266 3.784028
12678271 2.588914
12678279 5.373401
12678281 5.871158

Total number of rows: 26773

Table truncated, full table size 467 Kbytes.




Supplementary file Size Download File type/resource
GSM3466987_F_4TGC.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap