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Sample GSM3466985 Query DataSets for GSM3466985
Status Public on Dec 31, 2019
Title primary bovine trophoblast giant cells from cow 3
Sample type RNA
Source name primary bovine trophoblast giant cells
Organism Bos taurus
Characteristics cell type: primary bovine trophoblast giant cells
Treatment protocol Primary UTC and TGC isolates were analyzed without further treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using the NucleoSpin RNA Kit including removal of genomic DNA with RNAse free rDNAse (Macherey-Nagel).
Label Biotin
Label protocol RNA from each cell sample was used to generate single strand DNA (Ambion WT Expression Kit). Fragmentation and labeling was performed with the Affymetrix Gene Chip WT Terminal Labeling and Hybridization Kit
Hybridization protocol The RNA was hybridized overnight (45°C) in the GeneChipR Hybridization Oven (Affymetrix) to the Affymetrix Bovine Gene 1.0 ST arrays. Washing and staining were performed according to the Affymetrix standard protocol.
Scan protocol The scanning of the microarray was done with the GeneChip Scanner 3000 7G (Affymetrix) and image processing was done with the software AGCC (Affymetrix).
Data processing Raw data were processed with the Expression Console (V1.4.1.46; Affymetrix), where normalization, background reduction and a gene-level summary was done using the RMA method (Robust Multichip Average).
Submission date Nov 13, 2018
Last update date Jan 24, 2020
Contact name Rainer Fuerbass
Organization name Leibniz Institute for Farm Animal Biology
Street address Wilhelm-Stahl-Allee
City Dummerstorf
ZIP/Postal code 18196
Country Germany
Platform ID GPL16500
Series (1)
GSE122474 Transcriptome analysis of bovine uninucleated trophoblast cells (UTC) and Trophoblast Giant Cells (TGC)

Data table header descriptions
VALUE log2 RMA signal

Data table
12678162 2.313869
12678166 3.05968
12678175 1.718094
12678178 3.754075
12678183 3.818111
12678186 3.790996
12678189 7.18933
12678219 1.901937
12678223 3.579302
12678232 5.003836
12678235 4.182929
12678240 4.033743
12678244 7.789095
12678246 6.048679
12678251 3.554814
12678254 4.387312
12678266 3.864891
12678271 2.499238
12678279 5.563873
12678281 6.081756

Total number of rows: 26773

Table truncated, full table size 467 Kbytes.

Supplementary file Size Download File type/resource
GSM3466985_F_3TGC.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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