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Sample GSM3466983 Query DataSets for GSM3466983
Status Public on Dec 31, 2019
Title primary bovine trophoblast giant cells from cow 2
Sample type RNA
Source name primary bovine trophoblast giant cells
Organism Bos taurus
Characteristics cell type: primary bovine trophoblast giant cells
Treatment protocol Primary UTC and TGC isolates were analyzed without further treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using the NucleoSpin RNA Kit including removal of genomic DNA with RNAse free rDNAse (Macherey-Nagel).
Label Biotin
Label protocol RNA from each cell sample was used to generate single strand DNA (Ambion WT Expression Kit). Fragmentation and labeling was performed with the Affymetrix Gene Chip WT Terminal Labeling and Hybridization Kit
Hybridization protocol The RNA was hybridized overnight (45°C) in the GeneChipR Hybridization Oven (Affymetrix) to the Affymetrix Bovine Gene 1.0 ST arrays. Washing and staining were performed according to the Affymetrix standard protocol.
Scan protocol The scanning of the microarray was done with the GeneChip Scanner 3000 7G (Affymetrix) and image processing was done with the software AGCC (Affymetrix).
Data processing Raw data were processed with the Expression Console (V1.4.1.46; Affymetrix), where normalization, background reduction and a gene-level summary was done using the RMA method (Robust Multichip Average).
Submission date Nov 13, 2018
Last update date Jan 24, 2020
Contact name Rainer Fuerbass
Organization name Leibniz Institute for Farm Animal Biology
Street address Wilhelm-Stahl-Allee
City Dummerstorf
ZIP/Postal code 18196
Country Germany
Platform ID GPL16500
Series (1)
GSE122474 Transcriptome analysis of bovine uninucleated trophoblast cells (UTC) and Trophoblast Giant Cells (TGC)

Data table header descriptions
VALUE log2 RMA signal

Data table
12678162 2.276653
12678166 3.134192
12678175 1.598413
12678178 3.776423
12678183 3.722938
12678186 3.806032
12678189 6.386354
12678219 2.085089
12678223 3.50711
12678232 4.406876
12678235 3.655753
12678240 3.81723
12678244 8.666837
12678246 5.865972
12678251 3.31971
12678254 4.606712
12678266 5.752353
12678271 2.478725
12678279 5.250742
12678281 5.929602

Total number of rows: 26773

Table truncated, full table size 467 Kbytes.

Supplementary file Size Download File type/resource
GSM3466983_F_2TGC.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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