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Sample GSM344216 Query DataSets for GSM344216
Status Public on Nov 21, 2008
Title NTHi 3
Sample type RNA
 
Source name NTHi lysate treated
Organism Mus musculus
Characteristics 2h treatment of MLE-15 cells
Treatment protocol MLE-15 cells were treated with sham (PBS), NTHi lysate (100 ug/ml) or EF2505-III (40 ug/ml) for 2 hours.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description NTHi lysate treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Nov 20, 2008
Last update date Nov 20, 2008
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6105
Series (1)
GSE13685 Gene expression in respiratory epithelial cells treated to induce StIR

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2417611 91.02023 40 3.726488 0.8608859
ILMN_1238136 107.1215 31 5.537067 0.3094198
ILMN_2721178 190.6376 31 10.59609 0.009981285
ILMN_2458840 111.3638 55 3.890221 0.2083593
ILMN_1230777 10058.69 38 271.4048 0
ILMN_2537239 6376.106 42 187.2651 0
ILMN_1246069 6889.809 53 128.3971 0
ILMN_2524361 192.8616 41 7.8476 0.009981285
ILMN_1243193 5610.068 37 120.3903 0
ILMN_1232042 100.4675 40 4.264703 0.5458515
ILMN_1242440 107.859 37 4.188105 0.2838428
ILMN_2543689 109.7791 43 4.752697 0.2370555
ILMN_1233188 93.49166 37 2.727546 0.7910168
ILMN_1224596 1040.92 41 28.92721 0
ILMN_1233643 100.7207 35 3.6582 0.5333749
ILMN_1222139 547.3604 31 18.30089 0.0006238303
ILMN_1259789 312.5942 40 10.51572 0.003119152
ILMN_2773981 2079.741 43 76.5071 0
ILMN_2771349 2097.122 45 115.9391 0
ILMN_2634566 3637.711 44 87.32275 0

Total number of rows: 46632

Table truncated, full table size 1941 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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