|
| Status |
Public on Dec 15, 2008 |
| Title |
hMSCs ctrl_1 |
| Sample type |
RNA |
| |
|
| Source name |
Human primary bone marrow mesenchymal stromal cells
|
| Organism |
Homo sapiens |
| Characteristics |
Patient 294
Female 69 years old
Obtained after informed consent during orthopedia hip surgery
Protocol approved by ethics committee of the Montreal Jewish General Hospital
|
| Biomaterial provider |
Our laboratory
|
| Treatment protocol |
Untreated
|
| Growth protocol |
Growth in MEM alpha medium with 20% FBS, passaged q 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells homogenized with Qiashredder homogenizer, RNA extracted with RNeasy Mini Kit. RNA verified with NanoDrop ND-1000, Agilent RNA 6000 Nano Assay and agarose electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
AminoAllyl aRNA labeling. The reactive amino group of 5-(3-aminoallyl)-UTP/5-(3-aminoallyl)-dUTP was used to conjugate the purified aRNA with the NHS-CyDye.
|
| |
|
| Hybridization protocol |
Phalanx Hybridization Protocol
|
| Scan protocol |
Phalanx scanning protocol
|
| Description |
Control cells
|
| Data processing |
Quantile normalization
|
| |
|
| Submission date |
Nov 19, 2008 |
| Last update date |
Nov 21, 2008 |
| Contact name |
Philippe M Campeau |
| E-mail(s) |
pcampeau@hotmail.com, philippe.campeau@mail.mcgill.ca
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Lab |
Brendan Lee Lab
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6254 |
| Series (1) |
| GSE13675 |
Conduritol B Epoxide treatment of MSCs and 293T cells |
|
