|
| Status |
Public on Sep 21, 2018 |
| Title |
Bone marrow E |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: bone marrow cells
age: 30 years old
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Using standard operating procedures, mononuclear cells from 20 healthy donors' bone marrow aspirates were isolated using Ficoll density gradient separation and cryopreserved in 90% FBS/ 10% DMSO for storage in 72 liquid nitrogen.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
scRNAseq was performed using 10X Genomics Single Cell 3’ Solution, version 2 according to manufacturer’s instructions (protocol rev A).
Droplet-based scRNAseq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Data were analyzed using Cell Ranger V2.0.0 (10X Genomics).
Genome_build: GRCh38
Supplementary_files_format_and_content: Cell Ranger generated one mtx file and two tsv files for gene ID and cell barcode ID.
|
| |
|
| Submission date |
Sep 19, 2018 |
| Last update date |
Sep 24, 2018 |
| Contact name |
Laura Dillon |
| E-mail(s) |
laura.dillon2@nih.gov
|
| Organization name |
National Heart Lung and Blood Institute, NIH
|
| Department |
Hematology Branch
|
| Lab |
Myeloid Malignancies
|
| Street address |
Room 10CRC 6-5142, 10 Center Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (2) |
| GSE120221 |
Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry [scRNA] |
| GSE120446 |
Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry |
|
| Relations |
| BioSample |
SAMN10095267 |
| SRA |
SRX4720042 |
