|
| Status |
Public on Sep 20, 2018 |
| Title |
E7_ST2neg-transfer_Nb_lung_eo_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
eosinophils in lungs
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
cell type: eosinophils
strain: BALB/c
|
| Growth protocol |
Prior to the infection, we transferred ST2- memory Th2 cells from mice 40 days after infection of 500 organisms of Larvae of Nippostrongylus brasiliensis (Nb). One month later, the mice were infected with 500 organisms of the Larvae. We sorted eosinophils from the lungs of the mice 4 days after the infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
|
| |
|
| Submission date |
Sep 19, 2018 |
| Last update date |
Sep 24, 2018 |
| Contact name |
Atsushi Onodera |
| Organization name |
Chiba University
|
| Department |
Immunology
|
| Street address |
Chuo-ku Inohana 1-8-1
|
| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE120173 |
Characterization of ST2+ and ST2- mTh cells in helminth infection |
|
| Relations |
| BioSample |
SAMN10091180 |
| SRA |
SRX4715148 |
