|
| Status |
Public on Nov 14, 2008 |
| Title |
D1_IP3: Drd1a Medium spiny neurons, dissected mouse striatum |
| Sample type |
RNA |
| |
|
| Source name |
Drd1a Medium spiny neurons, dissected mouse striatum
|
| Organism |
Mus musculus |
| Characteristics |
TRAP RNA
Driver: Drd1a, Line: CP73, Region: striatum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRAP affinity purification of ribosomes from BAC transgenic mice (see accompanying manuscript), followed by Trizol-LS extraction, DNAse treatment, and RNeasy-Minelute Cleanup.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix two cycle amplification kit, starting with 15 ng of total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol for mouse 430 2.0 platform.
|
| Scan protocol |
Standard Affymetrix protocol for mouse 430 2.0 platform.
|
| Description |
D1_IP3
|
| Data processing |
In Genespring GX 7.3.1, data were normalized with the GC-RMA algorithm, and expression values on each chip were normalized to that chip’s 50th percentile.
Using the Limma package from Bioconductor project, data were converted to log2 scale.
|
| |
|
| Submission date |
Oct 28, 2008 |
| Last update date |
Oct 29, 2008 |
| Contact name |
Nathaniel Heintz |
| E-mail(s) |
heintz@rockefeller.edu
|
| Organization name |
HHMI/Rockefeller University
|
| Street address |
1230 York Ave, Box 260
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE13384 |
Comparative analysis of Drd1+ Medium Spiny Neurons and Drd2+ Medium Spiny Neurons |
| GSE13394 |
A translational profiling approach for the molecular characterization of CNS cell types |
|
