|
| Status |
Public on Dec 31, 2008 |
| Title |
INS_cells_2 |
| Sample type |
RNA |
| |
|
| Source name |
INS_cells_2
|
| Organism |
Rattus norvegicus |
| Characteristics |
clonal INS cells
Cultured insulinoma cell lines
|
| Growth protocol |
INS-1 cells were cultured in complete medium and collected for analysis after 48h. Primary rat beta-cells (>87% beta-cells) and non-beta-cells (<3% beta cells, mostly alpha) were isolated by FACS mediated purification of two different rat islet preparations.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
INS_cells_2
|
| Data processing |
Raw CEL intensity data were GCRMA normalized using R/Bioconductor
|
| |
|
| Submission date |
Oct 27, 2008 |
| Last update date |
Oct 30, 2008 |
| Contact name |
Burak Kutlu |
| E-mail(s) |
bkutlu@systemsbiology.org
|
| Phone |
206-732-1200
|
| Organization name |
Institute for Systems Biology
|
| Street address |
1441 North 34th Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE13381 |
Detailed transcriptome atlas of the pancreatic beta cell |
|
