|
| Status |
Public on Aug 31, 2019 |
| Title |
human patient blood mRNA 3 |
| Sample type |
RNA |
| |
|
| Source name |
human patient blood mRNA
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Chinese imported falciparum malaria patient
age (years): 32
gender: male
tissue: blood
parasitemia (%): 1.5%
concentration of hemoglobin (g/l): 176
wbc (×10^9/l): 4.12
platelets (×10^9/l): 73
lymphocytes (×10^9/l): 0.67
lymphocyte (%): 16.3
neutrophils (×10^9/l): 2.84
neutrophils (%): 69
monocytess (×10^9/l): 0.51
monocyte (%): 12.4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
mRNA expression
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 28, 2018 |
| Last update date |
Sep 01, 2019 |
| Contact name |
Yang Zou |
| E-mail(s) |
zouyang.bfh@outlook.com
|
| Organization name |
Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
|
| Street address |
Xicheng District road 95 Yongan
|
| City |
beijing |
| ZIP/Postal code |
100000 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE119150 |
Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [mRNA] |
| GSE119152 |
Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling |
|
