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Sample GSM334225 Query DataSets for GSM334225
Status Public on Jan 15, 2009
Title Populus balsamifera_1006_mature_leaf_midday_2
Sample type RNA
Source name Populus balsamifera, clone 1006, mature leaf, midday
Organism Populus balsamifera
Characteristics Mature leaf, including petiole - from 3 individuals
Alberta Pacific Forest Industries clone 1006
Clonally-propagated, 8-week-old Populus balsamifera saplings
Leaves collected in the middle of the light period
Biomaterial provider Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
Growth protocol Plant material was collected from clonally-propagated, 8-week-old Populus balsamifera saplings (1006, Alberta Pacific Forest Industries Inc., Boyle, AB, Canada) grown in a climate-controlled growth chamber. Saplings were grown in Sunshine Mix (Sun Gro Horticulture, Vancouver, BC, Canada) with a 16h photoperiod, a maximum daytime temperature of 22°C and a minimum nighttime temperature of 17°C. All tissues were collected at midday. All tissues were collected from saplings without water limitations. Mature leaf samples include the first fully expanded leaf including petiole. Each sample comprises pooled material from three individuals. All collected plant materials were flash frozen in liquid nitrogen upon harvesting and stored at -80°C until RNA was extracted.
Extracted molecule total RNA
Extraction protocol Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-11
Label biotin
Label protocol Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
Hybridization protocol Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
Submission date Oct 16, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
Phone 416-946-0817
Fax 416-978-5878
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
VALUE normalized (scaled) signal count data

Data table
AFFX-BioB-5_at 940.067 P 0.000389797
AFFX-BioB-M_at 1430.83 P 4.42873e-05
AFFX-BioB-3_at 932.558 P 4.42873e-05
AFFX-BioC-5_at 3282.95 P 5.16732e-05
AFFX-BioC-3_at 2472.43 P 4.42873e-05
AFFX-BioDn-5_at 4223.96 P 4.42873e-05
AFFX-BioDn-3_at 13559.7 P 4.42873e-05
AFFX-CreX-5_at 33311.9 P 5.16732e-05
AFFX-CreX-3_at 39193.9 P 4.42873e-05
AFFX-DapX-5_at 45.1538 A 0.0780018
AFFX-DapX-M_at 39.9908 A 0.48511
AFFX-DapX-3_at 6.85398 A 0.824693
AFFX-LysX-5_at 24.5209 A 0.51489
AFFX-LysX-M_at 5.18178 A 0.883887
AFFX-LysX-3_at 36.8012 A 0.327065
AFFX-PheX-5_at 4.66326 A 0.986189
AFFX-PheX-M_at 7.63039 A 0.686292
AFFX-PheX-3_at 78.1658 A 0.175328
AFFX-ThrX-5_at 13.6122 A 0.772383
AFFX-ThrX-M_at 20.4832 A 0.470241

Total number of rows: 61413

Table truncated, full table size 2516 Kbytes.

Supplementary file Size Download File type/resource
GSM334225.CEL.gz 4.6 Mb (ftp)(http) CEL
GSM334225.CHP.gz 334.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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