|
| Status |
Public on Feb 19, 2019 |
| Title |
BUC019_6N: Control_purified CD16+ neutrophils |
| Sample type |
genomic |
| |
|
| Source name |
Control_purified CD16+ neutrophils
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Control
gender: Female
tissue: purified CD16+ neutrophils
|
| Treatment protocol |
Peripheral blood mononuclear cells (PMBCs) were separated from red blood cells and polynuclear cells using Ficoll-Paque PLUS (GE Healthcare, Chicago, USA). Isolated PBMCs were sequentially incubated with anti-CD19, anti-CD8, and anti-CD4 microbeads (Miltenyi Biotec, Bergisch, Germany). Red blood cells and polynuclear cells were separated using 3% dextran (Fisher Scientific, Hampton, USA), as previously reported, and neutrophils were subsequently isolated using anti-CD16 microbeads (Miltenyi Biotec, Bergisch, Germany). The purity of each cell lineage was evaluated by flow cytometry.
|
| Growth protocol |
Blood samples were kept at room temperature and processed within 30 min of the blood draw
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from whole blood using FlexiGene DNA Kit according to the manufacturer’s protocol (Qiagen). DNA samples were then bisulfite converted using the EpiTect Bisulfite Kit according to the manufacturer’s protocol (Qiagen)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina labeling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Intensities were normalized using Illumina’s internal normalization probes and algorithms.
|
| |
|
| Submission date |
Aug 06, 2018 |
| Last update date |
Feb 20, 2019 |
| Contact name |
Brian Chung |
| E-mail(s) |
bhychung@hku.hk
|
| Organization name |
The University of Hong Kong
|
| Department |
Paediatrics and Adolescent Medicine
|
| Street address |
102 Pokfulam Road
|
| City |
Hong Kong |
| State/province |
Hong Kong |
| ZIP/Postal code |
852 |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE118144 |
Cell lineage-specific genome-wide DNA methylation analysis of patients with paediatric-onset systemic lupus erythematosus |
|
