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Sample GSM328274 Query DataSets for GSM328274
Status Public on Jan 31, 2009
Title Populus x canescens leaf hypoxia_rep_02
Sample type RNA
Source name poplar leaf tissue flooded up to 168h
Organism Populus tremula x Populus alba
Characteristics Experiments were performed with 3-month-old hybrid poplar plants (Populus x canescens). At the beginning of the experiment, the shoots of the trees had a size of 20 to 25 cm. To induce root hypoxia 52 trees were transferred into large plastic containers. The containers were filled with tap water until the water level exceeded the soil surface by 2-3 cm. Shoots were exposed to ambient air.
Extracted molecule total RNA
Extraction protocol For total RNA extraction the Plant RNeasy kit (Qiagen) was used according to the manufacturer’s instructions with some minor modifications. Aliquots of 90 mg of frozen plant powder were added to 900 µl RLT buffer supplemented with 1% ß-mercaptoethanol, 1% PVPP and 2% (w/v) polyethylenglycol (PEG 10,000), and then incubated for 10 min at 58°C. The samples were further processed according to the manufacturer’s protocol. Total RNA was treated with DNase during (RNase free DNase kit, Qiagen) and after (DNA-free TM kit, Ambion) the extraction procedure.
The quality of the isolated total RNA was tested using an Agilent Bioanalyzer and spectrophotometric analysis (NanoDrop® ND-1000, NanoDrop Technologies) to determine the A260 to A280 ratio.
Label Biotin
Label protocol Labelling of cRNA from 5 µg of leaf total RNA was performed exactly as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
Hybridization protocol standard Affymetrix protocol (see GeneChip Expression Analysis Technical Manual)
Scan protocol standard Affymetrix protocol (see GeneChip Expression Analysis Technical Manual)
Description Source leaves of Grey poplar 168 h hypoxia treated
Data processing GCOS1,4 (Affymetrix)
Submission date Oct 07, 2008
Last update date Oct 17, 2008
Contact name Juergen Kreuzwieser
Phone +49 761 203 8311
Fax +49 761 203 8302
Organization name University of Freiburg
Department Chair of Tree Physiology
Street address Georges-Koehler-Allee
City Freiburg
ZIP/Postal code 79110
Country Germany
Platform ID GPL4359
Series (1)
GSE13109 Effect of hypoxia on gene expression in Grey poplar

Data table header descriptions
VALUE Scaled expression value
ABS_CALL A qualitative measurement indicating if the probe set is detected (Present; P), not detected (Absent; A), or marginally detected (Marginal;M)
DETECTION P-VALUE A p-value indicating the significance of the Detection call

Data table
AFFX-BioB-5_at 1229.44 P 5.16732e-05
AFFX-BioB-M_at 1839.05 P 4.42873e-05
AFFX-BioB-3_at 1312.83 P 4.42873e-05
AFFX-BioC-5_at 3794.57 P 4.42873e-05
AFFX-BioC-3_at 3825.73 P 4.42873e-05
AFFX-BioDn-5_at 6922.06 P 4.42873e-05
AFFX-BioDn-3_at 14484.9 P 4.42873e-05
AFFX-CreX-5_at 40141.1 P 5.16732e-05
AFFX-CreX-3_at 44953 P 4.42873e-05
AFFX-DapX-5_at 657.263 P 5.16732e-05
AFFX-DapX-M_at 1438.67 P 6.02111e-05
AFFX-DapX-3_at 2236.74 P 5.16732e-05
AFFX-LysX-5_at 143.353 P 0.000753643
AFFX-LysX-M_at 221.019 P 0.00022441
AFFX-LysX-3_at 567.339 P 5.16732e-05
AFFX-PheX-5_at 166.01 P 0.000126798
AFFX-PheX-M_at 171.134 P 0.000126798
AFFX-PheX-3_at 239.951 P 0.000445901
AFFX-ThrX-5_at 95.0423 P 0.0200219
AFFX-ThrX-M_at 302.069 P 4.42873e-05

Total number of rows: 61413

Table truncated, full table size 2531 Kbytes.

Supplementary file Size Download File type/resource
GSM328274.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM328274.CHP.gz 340.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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