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Sample GSM328105 Query DataSets for GSM328105
Status Public on Jan 31, 2009
Title Populus x canescens leaf hypoxia_rep_01
Sample type RNA
Source name poplar leaf tissue flooded up to 168h
Organism Populus tremula x Populus alba
Characteristics Experiments were performed with 3-month-old hybrid poplar plants (Populus x canescens). At the beginning of the experiment, the shoots of the trees had a size of 20 to 25 cm. To induce root hypoxia 52 trees were transferred into large plastic containers. The containers were filled with tap water until the water level exceeded the soil surface by 2-3 cm. Shoots were exposed to ambient air.
Extracted molecule total RNA
Extraction protocol For total RNA extraction the Plant RNeasy kit (Qiagen) was used according to the manufacturer’s instructions with some minor modifications. Aliquots of 90 mg of frozen plant powder were added to 900 µl RLT buffer supplemented with 1% ß-mercaptoethanol, 1% PVPP and 2% (w/v) polyethylenglycol (PEG 10,000), and then incubated for 10 min at 58°C. The samples were further processed according to the manufacturer’s protocol. Total RNA was treated with DNase during (RNase free DNase kit, Qiagen) and after (DNA-free TM kit, Ambion) the extraction procedure.
The quality of the isolated total RNA was tested using an Agilent Bioanalyzer and spectrophotometric analysis (NanoDrop® ND-1000, NanoDrop Technologies) to determine the A260 to A280 ratio.
Label Biotin
Label protocol Labelling of cRNA from 5 µg of leaf total RNA was performed exactly as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
Hybridization protocol standard Affymetrix protocol (see GeneChip Expression Analysis Technical Manual)
Scan protocol standard Affymetrix protocol (see GeneChip Expression Analysis Technical Manual)
Description Source leaves of Grey poplar 168 h hypoxia
Data processing GCOS1,4 (Affymetrix)
Submission date Oct 07, 2008
Last update date Oct 17, 2008
Contact name Juergen Kreuzwieser
Phone +49 761 203 8311
Fax +49 761 203 8302
Organization name University of Freiburg
Department Chair of Tree Physiology
Street address Georges-Koehler-Allee
City Freiburg
ZIP/Postal code 79110
Country Germany
Platform ID GPL4359
Series (1)
GSE13109 Effect of hypoxia on gene expression in Grey poplar

Data table header descriptions
VALUE Scaled expression value
ABS_CALL A qualitative measurement indicating if the probe set is detected (Present; P), not detected (Absent; A), or marginally detected (Marginal;M)
DETECTION P-VALUE A p-value indicating the significance of the Detection call

Data table
AFFX-BioB-5_at 1735.1 P 5.16732e-05
AFFX-BioB-M_at 2529.95 P 4.42873e-05
AFFX-BioB-3_at 1577.58 P 4.42873e-05
AFFX-BioC-5_at 4599.34 P 5.16732e-05
AFFX-BioC-3_at 4813.57 P 4.42873e-05
AFFX-BioDn-5_at 9672.7 P 4.42873e-05
AFFX-BioDn-3_at 18071.9 P 4.42873e-05
AFFX-CreX-5_at 51290 P 5.16732e-05
AFFX-CreX-3_at 59253.8 P 4.42873e-05
AFFX-DapX-5_at 27647.7 P 4.42873e-05
AFFX-DapX-M_at 39235.6 P 4.42873e-05
AFFX-DapX-3_at 44735.9 P 4.42873e-05
AFFX-LysX-5_at 3880.93 P 4.42873e-05
AFFX-LysX-M_at 6230.64 P 4.42873e-05
AFFX-LysX-3_at 14579.4 P 4.42873e-05
AFFX-PheX-5_at 7296.78 P 4.42873e-05
AFFX-PheX-M_at 10032.7 P 4.42873e-05
AFFX-PheX-3_at 7829.04 P 4.42873e-05
AFFX-ThrX-5_at 9104.28 P 5.16732e-05
AFFX-ThrX-M_at 13527.9 P 4.42873e-05

Total number of rows: 61413

Table truncated, full table size 2524 Kbytes.

Supplementary file Size Download File type/resource
GSM328105.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM328105.CHP.gz 336.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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