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Sample GSM327661 Query DataSets for GSM327661
Status Public on Jan 15, 2009
Title Populus balsamifera_Male_Catkin_midday_3
Sample type RNA
 
Source name Populus balsamifera, Male catkin, midday
Organism Populus balsamifera
Characteristics Male Catkin
Alberta Pacific Forest Industries
Field trial in Grassland, AB, Canada
Collected April 30, 2007
Biomaterial provider Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
Growth protocol Catkins were collected from mature P. balsamifera trees grown by Alberta Pacific Forest Industries Inc. in a field trial in Grassland, AB, Canada on April 30, 2007. Material was flash frozen in liquid nitrogen and then stored at -80┬░C until RNA was extracted.
Extracted molecule total RNA
Extraction protocol Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-116.
Label biotin
Label protocol Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
 
Hybridization protocol Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
 
Submission date Oct 07, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
E-mail(s) malcolm.campbell@utoronto.ca
Phone 416-946-0817
Fax 416-978-5878
URL http://www.csb.utoronto.ca/faculty/campbell-malcolm
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
 
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
ID_REF
VALUE normalized (scaled) signal count data
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 3060.12 P 0.000195116
AFFX-BioB-M_at 4230.81 P 8.14279e-05
AFFX-BioB-3_at 2696.86 P 0.000146581
AFFX-BioC-5_at 10013.9 P 7.00668e-05
AFFX-BioC-3_at 9723.48 P 5.16732e-05
AFFX-BioDn-5_at 16468.1 P 5.16732e-05
AFFX-BioDn-3_at 34901.7 P 4.42873e-05
AFFX-CreX-5_at 94668.3 P 5.16732e-05
AFFX-CreX-3_at 116443 P 4.42873e-05
AFFX-DapX-5_at 8020.51 P 0.00556451
AFFX-DapX-M_at 35376.7 P 8.14279e-05
AFFX-DapX-3_at 56423.3 P 0.000753643
AFFX-LysX-5_at 1367.64 P 0.000972149
AFFX-LysX-M_at 2673.53 P 0.000445901
AFFX-LysX-3_at 3306.78 P 0.0219019
AFFX-PheX-5_at 2626.57 P 0.000753643
AFFX-PheX-M_at 7157.79 P 0.000224668
AFFX-PheX-3_at 3225.46 P 0.036569
AFFX-ThrX-5_at 2810.53 P 0.0151826
AFFX-ThrX-M_at 7066.32 P 0.000856509

Total number of rows: 61413

Table truncated, full table size 2470 Kbytes.




Supplementary file Size Download File type/resource
GSM327661.CEL.gz 4.5 Mb (ftp)(http) CEL
GSM327661.CHP.gz 333.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Raw data provided as supplementary file
Processed data provided as supplementary file

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