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Sample GSM327659 Query DataSets for GSM327659
Status Public on Jan 15, 2009
Title Populus balsamifera_Male_Catkin_midday_1
Sample type RNA
 
Source name Populus balsamifera, Male catkin, midday
Organism Populus balsamifera
Characteristics Male Catkin
Alberta Pacific Forest Industries
Field trial in Grassland, AB, Canada
Collected April 30, 2007
Biomaterial provider Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
Growth protocol Catkins were collected from mature P. balsamifera trees grown by Alberta Pacific Forest Industries Inc. in a field trial in Grassland, AB, Canada on April 30, 2007. Material was flash frozen in liquid nitrogen and then stored at -80┬░C until RNA was extracted.
Extracted molecule total RNA
Extraction protocol Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-116.
Label biotin
Label protocol Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
 
Hybridization protocol Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
 
Submission date Oct 07, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
E-mail(s) malcolm.campbell@utoronto.ca
Phone 416-946-0817
Fax 416-978-5878
URL http://www.csb.utoronto.ca/faculty/campbell-malcolm
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
 
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
ID_REF
VALUE normalized (scaled) signal count data
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 2601.84 P 5.16732e-05
AFFX-BioB-M_at 5006.97 P 5.16732e-05
AFFX-BioB-3_at 3410.74 P 5.16732e-05
AFFX-BioC-5_at 9442.32 P 4.42873e-05
AFFX-BioC-3_at 9372.27 P 4.42873e-05
AFFX-BioDn-5_at 15850.1 P 4.42873e-05
AFFX-BioDn-3_at 35457.8 P 4.42873e-05
AFFX-CreX-5_at 87549 P 5.16732e-05
AFFX-CreX-3_at 105664 P 4.42873e-05
AFFX-DapX-5_at 12752.3 P 4.42873e-05
AFFX-DapX-M_at 29392.8 P 4.42873e-05
AFFX-DapX-3_at 50474.9 P 4.42873e-05
AFFX-LysX-5_at 1406.75 P 4.42246e-05
AFFX-LysX-M_at 4527.34 P 7.00668e-05
AFFX-LysX-3_at 8471.35 P 4.42873e-05
AFFX-PheX-5_at 3680.86 P 4.42873e-05
AFFX-PheX-M_at 6631.43 P 6.02111e-05
AFFX-PheX-3_at 5834.73 P 0.000126798
AFFX-ThrX-5_at 3532.29 P 0.000169227
AFFX-ThrX-M_at 6538.47 P 5.16732e-05

Total number of rows: 61413

Table truncated, full table size 2488 Kbytes.




Supplementary file Size Download File type/resource
GSM327659.CEL.gz 4.2 Mb (ftp)(http) CEL
GSM327659.CHP.gz 337.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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