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Sample GSM327657 Query DataSets for GSM327657
Status Public on Jan 15, 2009
Title Populus balsamifera_Female_Catkin_midday_2
Sample type RNA
Source name Populus balsamifera, Female catkin, midday
Organism Populus balsamifera
Characteristics Female Catkin
Alberta Pacific Forest Industries
Field trial in Grassland, AB, Canada
Collected April 30, 2007
Biomaterial provider Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
Growth protocol Catkins were collected from mature P. balsamifera trees grown by Alberta Pacific Forest Industries Inc. in a field trial in Grassland, AB, Canada on April 30, 2007. Material was flash frozen in liquid nitrogen and then stored at -80┬░C until RNA was extracted.
Extracted molecule total RNA
Extraction protocol Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-116.
Label biotin
Label protocol Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
Hybridization protocol Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
Submission date Oct 07, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
Phone 416-946-0817
Fax 416-978-5878
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
VALUE normalized (scaled) signal count data

Data table
AFFX-BioB-5_at 1762.53 P 0.00010954
AFFX-BioB-M_at 2851.71 P 4.42873e-05
AFFX-BioB-3_at 2519.9 P 4.42873e-05
AFFX-BioC-5_at 7295.51 P 5.16732e-05
AFFX-BioC-3_at 7045.36 P 4.42873e-05
AFFX-BioDn-5_at 11685.4 P 4.42873e-05
AFFX-BioDn-3_at 26466.6 P 4.42873e-05
AFFX-CreX-5_at 67052 P 5.16732e-05
AFFX-CreX-3_at 81629.4 P 4.42873e-05
AFFX-DapX-5_at 8469.92 P 0.002867
AFFX-DapX-M_at 22777.1 P 5.16732e-05
AFFX-DapX-3_at 40169.5 P 4.42873e-05
AFFX-LysX-5_at 1037.59 P 0.000146581
AFFX-LysX-M_at 2455.82 P 0.000340305
AFFX-LysX-3_at 4111.27 P 0.000146581
AFFX-PheX-5_at 1732.91 P 0.000258358
AFFX-PheX-M_at 4958.89 P 0.000126798
AFFX-PheX-3_at 3980.99 P 0.0113844
AFFX-ThrX-5_at 2160.15 P 0.0012475
AFFX-ThrX-M_at 4387.67 P 9.4506e-05

Total number of rows: 61413

Table truncated, full table size 2485 Kbytes.

Supplementary file Size Download File type/resource
GSM327657.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM327657.CHP.gz 335.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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