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Sample GSM327656 Query DataSets for GSM327656
Status Public on Jan 15, 2009
Title Populus balsamifera_Female_Catkin_midday_1
Sample type RNA
 
Source name Populus balsamifera, female catkin, midday
Organism Populus balsamifera
Characteristics Female Catkin
Alberta Pacific Forest Industries
Field trial in Grassland, AB, Canada
Collected April 30, 2007
Biomaterial provider Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
Growth protocol Catkins were collected from mature P. balsamifera trees grown by Alberta Pacific Forest Industries Inc. in a field trial in Grassland, AB, Canada on April 30, 2007. Material was flash frozen in liquid nitrogen and then stored at -80┬░C until RNA was extracted.
Extracted molecule total RNA
Extraction protocol Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-116.
Label biotin
Label protocol Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
 
Hybridization protocol Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
 
Submission date Oct 07, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
E-mail(s) malcolm.campbell@utoronto.ca
Phone 416-946-0817
Fax 416-978-5878
URL http://www.csb.utoronto.ca/faculty/campbell-malcolm
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
 
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
ID_REF
VALUE normalized (scaled) signal count data
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1331.56 P 0.000224668
AFFX-BioB-M_at 2569.15 P 6.02111e-05
AFFX-BioB-3_at 1985.43 P 6.02111e-05
AFFX-BioC-5_at 5558.02 P 5.16732e-05
AFFX-BioC-3_at 5185.05 P 4.42873e-05
AFFX-BioDn-5_at 9321.96 P 4.42873e-05
AFFX-BioDn-3_at 20373.7 P 4.42873e-05
AFFX-CreX-5_at 55325.2 P 4.42873e-05
AFFX-CreX-3_at 66637.3 P 4.42873e-05
AFFX-DapX-5_at 12473 P 7.00668e-05
AFFX-DapX-M_at 24843.5 P 4.42873e-05
AFFX-DapX-3_at 40933.9 P 7.00668e-05
AFFX-LysX-5_at 1132.13 P 8.14279e-05
AFFX-LysX-M_at 3043.35 P 8.14279e-05
AFFX-LysX-3_at 4624.46 P 8.14279e-05
AFFX-PheX-5_at 2141.37 P 0.000296708
AFFX-PheX-M_at 5866.02 P 7.00668e-05
AFFX-PheX-3_at 5127.3 P 0.00255552
AFFX-ThrX-5_at 2363.42 P 0.000224668
AFFX-ThrX-M_at 6121.07 P 4.42873e-05

Total number of rows: 61413

Table truncated, full table size 2487 Kbytes.




Supplementary file Size Download File type/resource
GSM327656.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM327656.CHP.gz 337.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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