|
| Status |
Public on Jan 15, 2009 |
| Title |
Populus balsamifera_1006_differentiating_xylem_midday_2 |
| Sample type |
RNA |
| |
|
| Source name |
Populus balsamifera, clone 1006, differentiating xylem, midday
|
| Organism |
Populus balsamifera |
| Characteristics |
Differentiating xylem – bottom 15 cm of stem - peel off bark and immediately scrape the surface of the exposed wood into micro collection tubes containing liquid nitrogen
Alberta Pacific Forest Industries clone 1006
Clonally-propagated, 8-week-old Populus balsamifera saplings
Differentiating xylem collected in the middle of the light period
|
| Biomaterial provider |
Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
|
| Growth protocol |
Plant material was collected from clonally-propagated, 8-week-old Populus balsamifera saplings (1006, Alberta Pacific Forest Industries Inc., Boyle, AB, Canada) grown in a climate-controlled growth chamber. Saplings were grown in Sunshine Mix (Sun Gro Horticulture, Vancouver, BC, Canada) with a 16h photoperiod, a maximum daytime temperature of 22°C and a minimum nighttime temperature of 17°C. All tissues were collected at midday. All tissues were collected from saplings without water limitations. Differentiating xylem was collected from the bottom 6 inches of the stem by peeling off the bark and immediately scraping the surface of the exposed wood into micro collection tubes containing liquid nitrogen. Flash frozen plant tissue was stored at -80°C until RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-116.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
|
| |
|
| Hybridization protocol |
Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
|
| Scan protocol |
Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
|
| Description |
Affymetrix protol: Fluidics script: EukGE-WS2v5
|
| Data processing |
MAS5.0, scaled to 500
|
| |
|
| Submission date |
Oct 06, 2008 |
| Last update date |
Dec 16, 2008 |
| Contact name |
Malcolm M. Campbell |
| E-mail(s) |
malcolm.campbell@utoronto.ca
|
| Phone |
416-946-0817
|
| Fax |
416-978-5878
|
| URL |
http://www.csb.utoronto.ca/faculty/campbell-malcolm
|
| Organization name |
University of Toronto
|
| Department |
Cell & Systems Biology
|
| Lab |
3058ESC
|
| Street address |
25 Willcocks Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S 3B2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE13990 |
Populus balsamifera developmental tissue series |
|
