|
| Status |
Public on Jan 15, 2009 |
| Title |
Populus balsamifera_1006_root_midday_3 |
| Sample type |
RNA |
| |
|
| Source name |
Populus balsamifera, clone 1006, root, midday
|
| Organism |
Populus balsamifera |
| Characteristics |
Distal 15cm of well-rinsed root mass
Alberta Pacific Forest Industries clone 1006
Clonally-propagated, 8-week-old Populus balsamifera saplings
Roots collected in the middle of the light period
|
| Biomaterial provider |
Alberta Pacific Forest Industries Inc., Boyle, AB, Canada
|
| Growth protocol |
Plant material was collected from clonally-propagated, 8-week-old Populus balsamifera saplings (1006, Alberta Pacific Forest Industries Inc., Boyle, AB, Canada) grown in a climate-controlled growth chamber. Saplings were grown in Sunshine Mix (Sun Gro Horticulture, Vancouver, BC, Canada) with a 16h photoperiod, a maximum daytime temperature of 22°C and a minimum nighttime temperature of 17°C. All tissues were collected at midday. All tissues were collected from saplings without water limitations. Root samples include the distal 15cm of well-rinsed roots mass. All collected plant materials were flash frozen in liquid nitrogen upon harvesting and stored at -80°C until RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Chang S, Puryear J, Cariney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter. 11:113-116.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
|
| |
|
| Hybridization protocol |
Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
|
| Scan protocol |
Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
|
| Description |
Affymetrix protol: Fluidics script: EukGE-WS2v5
|
| Data processing |
MAS5.0, scaled to 500
|
| |
|
| Submission date |
Oct 06, 2008 |
| Last update date |
Dec 16, 2008 |
| Contact name |
Malcolm M. Campbell |
| E-mail(s) |
malcolm.campbell@utoronto.ca
|
| Phone |
416-946-0817
|
| Fax |
416-978-5878
|
| URL |
http://www.csb.utoronto.ca/faculty/campbell-malcolm
|
| Organization name |
University of Toronto
|
| Department |
Cell & Systems Biology
|
| Lab |
3058ESC
|
| Street address |
25 Willcocks Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S 3B2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE13990 |
Populus balsamifera developmental tissue series |
|
