GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM327403 Query DataSets for GSM327403
Status Public on Jan 15, 2009
Title Populus balsamifera_20071015_Etiolated_seedling_6d_3
Sample type RNA
Source name 6 day old Populus balsamifera seedlings germinated in continuous darkness for 6 days
Organism Populus balsamifera
Characteristics Whole seedling - 20 individuals
Seedlot number 20071015
6 days post germination
Continuous light
Biomaterial provider National Tree Seed Centre, Fredericton, NB, Canada
Growth protocol Seedlings were germinated in a continuously dark growth chamber on wetted filter paper in petri plates with a constant temperature of 21°C. They were grown in this way for 6 days. On the 6th day, whole seedlings were collected and were flash frozen in liquid nitrogen upon harvesting and stored at -80°C until RNA was extracted. Each sample comprises pooled material from 20 individuals.
Extracted molecule total RNA
Extraction protocol Trizol method (Invitrogen, Burlington, ON, Canada)
Label biotin
Label protocol Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay
Hybridization protocol Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
Submission date Oct 06, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
Phone 416-946-0817
Fax 416-978-5878
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
VALUE normalized (scaled) signal count data

Data table
AFFX-BioB-5_at 691.945 P 6.02111e-05
AFFX-BioB-M_at 1211.47 P 4.42873e-05
AFFX-BioB-3_at 799.741 P 4.42873e-05
AFFX-BioC-5_at 2560.86 P 5.16732e-05
AFFX-BioC-3_at 2439.57 P 4.42873e-05
AFFX-BioDn-5_at 6034.56 P 4.42873e-05
AFFX-BioDn-3_at 10685.7 P 4.42873e-05
AFFX-CreX-5_at 30634.1 P 5.16732e-05
AFFX-CreX-3_at 34477.1 P 4.42873e-05
AFFX-DapX-5_at 19.9364 A 0.5
AFFX-DapX-M_at 9.87646 A 0.617401
AFFX-DapX-3_at 20.3773 A 0.48511
AFFX-LysX-5_at 17.6832 A 0.559379
AFFX-LysX-M_at 19.7392 A 0.737191
AFFX-LysX-3_at 58.0889 A 0.102165
AFFX-PheX-5_at 64.6284 M 0.050229
AFFX-PheX-M_at 6.6449 A 0.617401
AFFX-PheX-3_at 4.00334 A 0.976071
AFFX-ThrX-5_at 9.93051 A 0.686277
AFFX-ThrX-M_at 35.3159 A 0.250796

Total number of rows: 61413

Table truncated, full table size 2497 Kbytes.

Supplementary file Size Download File type/resource
GSM327403.CEL.gz 4.3 Mb (ftp)(http) CEL
GSM327403.CHP.gz 343.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Raw data provided as supplementary file
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap