|
| Status |
Public on Jan 15, 2009 |
| Title |
Populus balsamifera_20071015_Etiolated_seedling_6d_2 |
| Sample type |
RNA |
| |
|
| Source name |
6 day old Populus balsamifera seedlings germinated in continuous darkness for 6 days
|
| Organism |
Populus balsamifera |
| Characteristics |
Whole seedling - 20 individuals
Seedlot number 20071015
6 days post germination
Continuous light
|
| Biomaterial provider |
National Tree Seed Centre, Fredericton, NB, Canada
|
| Growth protocol |
Seedlings were germinated in a continuously dark growth chamber on wetted filter paper in petri plates with a constant temperature of 21°C. They were grown in this way for 6 days. On the 6th day, whole seedlings were collected and were flash frozen in liquid nitrogen upon harvesting and stored at -80°C until RNA was extracted. Each sample comprises pooled material from 20 individuals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method (Invitrogen, Burlington, ON, Canada)
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol: Eukaryotic sample and array processing: One-cycle target labeling assay
|
| |
|
| Hybridization protocol |
Affymetrix protocol: Eukaryotic sample and array processing: Eukaryotic target hybridization
|
| Scan protocol |
Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
|
| Description |
Affymetrix protocol: Fluidics script: EukGE-WS2v5
|
| Data processing |
MAS5.0, scaled to 500
|
| |
|
| Submission date |
Oct 06, 2008 |
| Last update date |
Dec 16, 2008 |
| Contact name |
Malcolm M. Campbell |
| E-mail(s) |
malcolm.campbell@utoronto.ca
|
| Phone |
416-946-0817
|
| Fax |
416-978-5878
|
| URL |
http://www.csb.utoronto.ca/faculty/campbell-malcolm
|
| Organization name |
University of Toronto
|
| Department |
Cell & Systems Biology
|
| Lab |
3058ESC
|
| Street address |
25 Willcocks Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S 3B2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE13990 |
Populus balsamifera developmental tissue series |
|
