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Sample GSM327358 Query DataSets for GSM327358
Status Public on Jan 15, 2009
Title Populus balsamifera_20071015_Seedling_continuous_light_6d_2
Sample type RNA
Source name Populus balsamifera seedlings, 6 days old, contiuous light
Organism Populus balsamifera
Characteristics Whole seedling - 20 individuals
Seedlot number 20071015
6 days post germination
Continuous light
Biomaterial provider National Tree Seed Centre, Fredericton, NB, Canada
Growth protocol Seedlings were germinated in a continuous light growth chamber on wetted filter paper in petri plates with a constant temperature of 21°C. They were grown in this way for five days. On the 6th day, whole seedlings were collected and were flash frozen in liquid nitrogen upon harvesting and stored at -80°C until RNA was extracted. Each sample comprises pooled material from 20 individuals.
Extracted molecule total RNA
Extraction protocol Trizol method (Invitrogen, Burlington, ON, Canada)
Label biotin
Label protocol Affymetrix protocol: Eukaryotic sample and array processing: One-cycle target labeling assay
Hybridization protocol Affymetrix protocol: Eukaryotic sample and array processing: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protocol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
Submission date Oct 06, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
Phone 416-946-0817
Fax 416-978-5878
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
VALUE normalized (scaled) signal count data

Data table
AFFX-BioB-5_at 572.265 P 0.000146581
AFFX-BioB-M_at 752.51 P 4.42873e-05
AFFX-BioB-3_at 566.574 P 4.42873e-05
AFFX-BioC-5_at 1882.07 P 5.16732e-05
AFFX-BioC-3_at 1841.98 P 4.42873e-05
AFFX-BioDn-5_at 3291.63 P 4.42873e-05
AFFX-BioDn-3_at 5825.29 P 4.42873e-05
AFFX-CreX-5_at 18337.1 P 5.16732e-05
AFFX-CreX-3_at 23537.7 P 4.42873e-05
AFFX-DapX-5_at 23957 P 0.000340305
AFFX-DapX-M_at 34138 P 0.000195116
AFFX-DapX-3_at 39928 P 4.42873e-05
AFFX-LysX-5_at 6642.36 P 0.00359458
AFFX-LysX-M_at 9758.38 P 0.000753643
AFFX-LysX-3_at 20886.5 P 5.16732e-05
AFFX-PheX-5_at 11234 P 7.00668e-05
AFFX-PheX-M_at 18244.8 P 8.14279e-05
AFFX-PheX-3_at 14751.8 P 0.000753643
AFFX-ThrX-5_at 8023.96 P 6.02111e-05
AFFX-ThrX-M_at 16043.2 P 4.42873e-05

Total number of rows: 61413

Table truncated, full table size 2506 Kbytes.

Supplementary file Size Download File type/resource
GSM327358.CEL.gz 5.0 Mb (ftp)(http) CEL
GSM327358.CHP.gz 334.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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