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Sample GSM327281 Query DataSets for GSM327281
Status Public on Jan 15, 2009
Title Populus balsamifera_20071015_Seedling_continuous_light_6d_1
Sample type RNA
Source name Populus balsamifera seedlings, 6 days old, contiuous light
Organism Populus balsamifera
Characteristics Whole seedling - 20 individuals
Seedlot number 20071015
6 days post germination
Continuous light
Biomaterial provider National Tree Seed Centre, Fredericton, NB, Canada
Growth protocol Seedlings were germinated in a continuous light growth chamber on wetted filter paper in petri plates with a constant temperature of 21°C. They were grown in this way for five days. On the 6th day, whole seedlings were collected and were flash frozen in liquid nitrogen upon harvesting and stored at -80°C until RNA was extracted. Each sample comprises pooled material from 20 individuals.
Extracted molecule total RNA
Extraction protocol Trizol method (Invitrogen, Burlington, ON, Canada)
Label biotin
Label protocol Affymetrix protocol: Eukaryotic sample and array processing: One-cycle target labeling assay
Hybridization protocol Affymetrix protocol: Eukaryotic sample and array processing: Eukaryotic target hybridization
Scan protocol Affymetrix protocol: GeneChip® Scanner 3000 7G Affymetrix 00-0073
Description Affymetrix protocol: Fluidics script: EukGE-WS2v5
Data processing MAS5.0, scaled to 500
Submission date Oct 06, 2008
Last update date Dec 16, 2008
Contact name Malcolm M. Campbell
Phone 416-946-0817
Fax 416-978-5878
Organization name University of Toronto
Department Cell & Systems Biology
Lab 3058ESC
Street address 25 Willcocks Street
City Toronto
State/province ON
ZIP/Postal code M5S 3B2
Country Canada
Platform ID GPL4359
Series (1)
GSE13990 Populus balsamifera developmental tissue series

Data table header descriptions
VALUE normalized (scaled) signal count data

Data table
AFFX-BioB-5_at 576.839 P 0.00010954
AFFX-BioB-M_at 1024.28 P 4.42873e-05
AFFX-BioB-3_at 581.339 P 7.00668e-05
AFFX-BioC-5_at 2467.38 P 7.00668e-05
AFFX-BioC-3_at 2672.39 P 5.16732e-05
AFFX-BioDn-5_at 4882.06 P 4.42873e-05
AFFX-BioDn-3_at 8714.23 P 4.42873e-05
AFFX-CreX-5_at 24173.5 P 5.16732e-05
AFFX-CreX-3_at 31886.9 P 4.42873e-05
AFFX-DapX-5_at 34440.4 P 0.000856509
AFFX-DapX-M_at 44015 P 0.000126798
AFFX-DapX-3_at 52821 P 4.42873e-05
AFFX-LysX-5_at 10972.4 P 6.02111e-05
AFFX-LysX-M_at 13865.2 P 0.0032123
AFFX-LysX-3_at 26366.5 P 7.00668e-05
AFFX-PheX-5_at 18753.8 P 4.42873e-05
AFFX-PheX-M_at 26235.4 P 5.16732e-05
AFFX-PheX-3_at 21552.5 P 0.000753643
AFFX-ThrX-5_at 11681.3 P 0.00762003
AFFX-ThrX-M_at 23151 P 0.000856509

Total number of rows: 61413

Table truncated, full table size 2497 Kbytes.

Supplementary file Size Download File type/resource
GSM327281.CEL.gz 5.0 Mb (ftp)(http) CEL
GSM327281.CHP.gz 333.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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