|
| Status |
Public on Nov 12, 2009 |
| Title |
Temporally restricted feeding in Liver of Cry1,Cry2 KO mice_CT38 |
| Sample type |
RNA |
| |
|
| Source name |
Cry1,Cry2 KO mouse Liver under restricted feeding at circadian time 38
|
| Organism |
Mus musculus |
| Characteristics |
Strain: Cry1,Cry2 double KO, Gender: female, Age: 8-16 weeks, Condition: temporally restricted food availability CT1(25)-CT9(33)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by Qiagen RNeasy columns
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix scan protocol
|
| Description |
Food was made available to Cry1,Cry2 double KO mice only during 8 hours of the day for two weeks. Mice were then released into constant darkness while food availability was still restricted to between CT25 and CT33 and tissue was collected during the second day of constant darkness
|
| Data processing |
Data from CEL files were normalized in R using the 'affy' package and the gcRMA algorithm. Log transformation of values was reversed and all values of a series of arrays were median normalized to 100
|
| |
|
| Submission date |
Oct 05, 2008 |
| Last update date |
Nov 12, 2009 |
| Contact name |
Christopher Vollmers |
| E-mail(s) |
vollmers@salk.edu
|
| URL |
http://www.salk.edu/faculty/faculty_details.php?id=65
|
| Organization name |
The Salk Institute for Biological Studies
|
| Department |
Regulatory Biology Laboratory
|
| Lab |
Panda Lab (RBIO-P)
|
| Street address |
10010 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE13062 |
The effects of temporally restricted feeding on hepatic gene expression of Cry1, Cry2 double KO mice |
| GSE13093 |
Feeding schedule and the circadian clock shape rhythms in hepatic gene expression |
|
