|
| Status |
Public on Nov 02, 2018 |
| Title |
PBMC_high_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
anhedonia: high
gender: male
age: 52
shaps_total: 16
race: Black/AA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells from heparine-collected peripheral blood were isolated with Ficoll-Paque® (GE Healthcare) protocol. Total RNA was isolated from isolated cells with RNeasy Plus Mini Kit (Qiagen). Quality control was performed with Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
RNAs were initially labeled into biotinylated cRNAs with the TargetAmp Nano Labeling Kit (Epicentre).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol on iScan microarray reader.
|
| Data processing |
Raw data were quantile-normalized using the Gene Expression Module v1.0 on GenomeStudio software v2011.1 (Illumina).
|
| |
|
| Submission date |
Jul 09, 2018 |
| Last update date |
Nov 03, 2018 |
| Contact name |
Margaret C Wardle |
| E-mail(s) |
Margaret.C.Wardle@uth.tmc.edu
|
| Phone |
7134862726
|
| Organization name |
The University of Texas Health Science Center at Houston
|
| Department |
Psychiatry and Behavioral Sciences
|
| Lab |
Center for Neurobehavioral Research on Addiciton
|
| Street address |
1941 East Rd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE116833 |
Peripheral gene expression in cocaine use disorders with low and high anhedonia |
|
