|
| Status |
Public on Sep 01, 2018 |
| Title |
US45103089_253949417602_S02_GE1_1100_51K1_1_1 |
| Sample type |
RNA |
| |
|
| Source name |
FFPE
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tumor Sample, advanced rectal adenocarcinoma
age at surgery: 70
dworak tumor regression grade: 3
gender: M
|
| Treatment protocol |
chemoradiation therapy, a combination of capecitabine (825 mg/m2) and radiations (50.4 Gy) provided in 28 fractions in 12 weeks. Clinical staging was accomplished using two physical examinations, endoscopic ultrasound (EUS) and nuclear magnetic resonance (NMR) before the administration of the neoadjuvant treatment. After 8 weeks from the completion of neoadjuvant therapy, surgery was performed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction
|
| Label |
Cy3
|
| Label protocol |
Agilent SureTag Labeling kit (Agilent Technologies, Palo Alto, CA, USA), enzymatically label 1.8 µg of amplified cDNA with Cyanine 3-dUTP for 2 hours at 37°C
|
| |
|
| Hybridization protocol |
Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray.
|
| Scan protocol |
Slides were scanned (Agilent SureScan Microarray Scanner) immediately after washing procedure according to the precedure outlined in Agilent one-color Microarray-based gene expression analysis manual.
|
| Description |
Gene Expression after capecitabine and radiotherapy cycle of 8 weeks
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software v10.7 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jul 06, 2018 |
| Last update date |
Sep 01, 2018 |
| Contact name |
Lorenzo Ferrando |
| E-mail(s) |
lorenzo.ferrando@edu.unige.it
|
| Organization name |
IRCCS - Ospedale Policlinico San Martino
|
| Department |
Department of Internal Medicine
|
| Lab |
Translational Laboratory
|
| Street address |
Viale Benedetto XV
|
| City |
Genova |
| ZIP/Postal code |
16132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE116742 |
Analysis of lncRNAs for the characterization of the response to neoadjuvant chemoradiotherapy |
|
